Phylogenetic and metabolic diversity of bacteria associated with cystic fibrosis
Phylogenetic and metabolic diversity of bacteria associated with cystic fibrosis
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DOI：
10.1038/ismej.2010.88
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发表时间：
2011-01-01
期刊：
影响因子：
11
通讯作者：
Cavanaugh, Colleen M.
中科院分区：
文献类型：
--
作者：
Guss, Adam M.;Roeselers, Guus;Cavanaugh, Colleen M.
在患有囊性纤维化（CF）的患者中，发病率和死亡率主要与慢性微生物支气管感染的不良后果有关，这种感染被认为是由一些机会性病原体引起的。然而，最近的证据表明其他微生物的存在，可能会显着影响感染的过程和结果。通过结合 16S rRNA 基因克隆文库、细菌培养和条形码 16S rRNA 扩增子的焦磷酸测序，对 CF 患者痰样本中存在的微生物群落进行了检查。除了先前识别的 CF 病原体（例如铜绿假单胞菌和金黄色葡萄球菌）之外，还检测到了 460 个通常与 CF 发病机制无关的系统发育多样化细菌属。每个样品中都存在来自多个细菌门的大量发酵兼性厌氧菌和专性厌氧菌。发现的许多细菌和序列是口咽微生物区系的正常居民，并且许多含有机会病原体。我们的数据表明，CF 肺内的这些采样不足的生物体是比通常假设的复杂得多的微生物生态系统的一部分。这些群落的表征是阐明不同细菌在疾病进展中的潜在作用并最终促进 CF 治疗进展的第一步。 ISME 期刊 (2011) 5, 20-29； doi：10.1038/ismej.2010.88； 2010 年 7 月 15 日在线发布
In patients afflicted with cystic fibrosis (CF), morbidity and mortality are primarily associated with the adverse consequences of chronic microbial bronchial infections, which are thought to be caused by a few opportunistic pathogens. However, recent evidence suggests the presence of other microorganisms, which may significantly affect the course and outcome of the infection. Using a combination of 16S rRNA gene clone libraries, bacterial culturing and pyrosequencing of barcoded 16S rRNA amplicons, the microbial communities present in CF patient sputum samples were examined. In addition to previously recognized CF pathogens such as Pseudomonas aeruginosa and Staphylococcus aureus, 460 phylogenetically diverse bacterial genera that are not typically associated with CF pathogenesis were also detected. A surprisingly large number of fermenting facultative and obligate anaerobes from multiple bacterial phyla was present in each sample. Many of the bacteria and sequences found were normal residents of the oropharyngeal microflora and with many containing opportunistic pathogens. Our data suggest that these undersampled organisms within the CF lung are part of a much more complex microbial ecosystem than is normally presumed. Characterization of these communities is the first step in elucidating potential roles of diverse bacteria in disease progression and to ultimately facilitate advances in CF therapy. The ISME Journal (2011) 5, 20-29; doi:10.1038/ismej.2010.88; published online 15 July 2010