Smartphone dongle for simultaneous measurement of hemoglobin concentration and detection of HIV antibodies
Smartphone dongle for simultaneous measurement of hemoglobin concentration and detection of HIV antibodies
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DOI：
10.1039/c5lc00609k
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发表时间：
2015-01-01
期刊：
影响因子：
6.1
通讯作者：
Sia, Samuel K.
中科院分区：
文献类型：
--
作者：
Guo, Tiffany;Patnaik, Ritish;Sia, Samuel K.
传统上很难将两类诊断测试合并到一个平台中。在这项工作中，我们展示了一种基于微流体的智能手机加密狗，它可以同时测量血红蛋白浓度并检测 HIV 抗体。具体来说，我们演示了如何将先前发表的测量金胶体上银沉淀的光密度的免疫测定装置扩展到通过比色测定定量测量血红蛋白浓度。通过用 CHAPS 洗涤剂裂解全血成分，我们使用该设备实现了血红蛋白浓度的高度可重复的测量。我们对来自哥伦比亚大学医学中心的 38 名患者样本进行了这项双重测试。与 Hemocue Hb 201+ 分析仪相比，我们设备的血红蛋白浓度准确度在 1.2 g dL(-1) 以内，而 HIV 免疫测定（在 CHAPS 去垢剂存在下）显示出 95% 的灵敏度和 95% 的特异性，与我们之前的研究相当。这项工作展示了将两类诊断测试（基于比色的定量测量和基于金胶体银沉淀的免疫测定）集成到与智能手机配合使用的低成本、快速、低功耗适配器中的可行性，并创建了一种具有临床实用性的新型双面板，可用于产前护理环境。
It is traditionally difficult to incorporate two classes of diagnostic tests into a single platform. In this work, we demonstrate a microfluidic-based smartphone dongle that simultaneously measures concentration of hemoglobin and detects HIV antibodies. Specifically, we demonstrate how a previously published immunoassay device, which measured optical density of silver precipitation on gold colloids, can be expanded to quantitatively measure hemoglobin concentration via a colorimetric assay. By lysing whole blood components with CHAPS detergent, we achieved highly reproducible measurement of hemoglobin concentration with the device. We tested this dual test on 38 patient samples from Columbia University Medical Center. Compared with the Hemocue Hb 201+ analyzer, hemoglobin concentrations from our device were accurate within 1.2 g dL(-1), while the HIV immunoassay (in the presence of CHAPS detergent) showed 95% sensitivity and 95% specificity, comparable to our previous studies. This work demonstrates the feasibility of integrating two classes of diagnostic tests (a colorimetric-based quantitative measurement and an immunoassay based on silver precipitation on gold colloids) into a low-cost, fast, and low-power dongle that works with smartphones, and creates a novel dual panel with clinical utility for antenatal-care settings.