B lymphocytes can be competent antigen-presenting cells for priming CD4+ T cells to protein antigens in vivo.
B lymphocytes can be competent antigen-presenting cells for priming CD4+ T cells to protein antigens in vivo.
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DOI：
10.4049/jimmunol.155.8.3734
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发表时间：
1995-10
影响因子：
4.4
通讯作者：
S. Constant;N. Schweitzer;J. West;Patricia Ranney;K. Bottomly
中科院分区：
文献类型：
--
作者：
S. Constant;N. Schweitzer;J. West;Patricia Ranney;K. Bottomly
研究了不同APC亚群在体内刺激初始CD 4 + T细胞产生肽和蛋白质Ag的潜在作用。缺乏B细胞的小鼠（microMT敲除小鼠）在其对蛋白质Ag而非肽Ag的引发中受损，表明在体内对蛋白质Ag的引发中需要B细胞。设计用于确定脾树突状细胞（DC）和B淋巴细胞在体内摄取肽或蛋白质Ag的能力的实验表明，肽Ag优先被DC摄取，而蛋白质在体内被Ag特异性B细胞摄取。进一步检查的Ag-特异性B细胞在体内脉冲与蛋白Ag揭示了一个显着的上调B7-2共刺激分子的表面表达，检测早在4小时后Ag管理。基于它们在蛋白Ag的摄取和加工中的效力以及它们通过Ag内化上调共刺激分子的能力，我们认为Ag特异性B细胞将是体内启动初始CD 4 + T细胞向蛋白Ag的重要APC。
The potential role of different subsets of APCs to stimulate naive CD4+ T cells to peptide and protein Ags in vivo was examined. Mice lacking B cells (microMT knockout mice) were impaired in their priming to protein but not peptide Ags, suggesting a requirement for B cells in priming to protein Ags in vivo. Experiments designed to determine the ability of splenic dendritic cells (DCs) and B lymphocytes to take up peptide or protein Ags in vivo demonstrated that peptide Ags were taken up preferentially by DCs, whereas proteins were taken up by Ag-specific B cells in vivo. A further examination of the Ag-specific B cells pulsed in vivo with protein Ags revealed a marked up-regulation in surface expression of B7-2 costimulatory molecules, detectable as early as 4 h after Ag administration. Based on their potency in the uptake and processing of protein Ags as well as their ability to up-regulate costimulatory molecules through Ag internalization, we suggest that Ag-specific B cells will be an important APC in priming naive CD4+ T cells to protein Ags in vivo.