An autonomously replicating transforming vector for Sulfolobus solfataricus
An autonomously replicating transforming vector for Sulfolobus solfataricus
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DOI：
10.1128/jb.180.12.3237-3240.1998
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发表时间：
1998-06-01
影响因子：
3.2
通讯作者：
Bartolucci, S
中科院分区：
文献类型：
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作者：
Cannio, R;Contursi, P;Bartolucci, S
通过插入到大肠杆菌中，构建了能够在硫磺硫化叶菌和大肠杆菌中转化并稳定维持的质粒。将病毒颗粒SSV 1的自主复制序列的大肠杆菌质粒和作为转化标记的hph（潮霉素磷酸转移酶）基因的合适突变体转化。该载体没有发生重排和/或染色体整合，并且其在硫化叶菌中的拷贝数通过将细胞暴露于丝裂霉素C而增加。
A plasmid able to transform and to be stably maintained both in Sulfolobus solfataricus and in Escherichia coli was constructed by insertion into an E. coli plasmid of the autonomously replicating sequence of the virus particle SSV1 and a suitable mutant of the hph (hygromycin phosphotransferase) gene as the transformation marker. The vector suffered no rearrangement and/or chromosome integration, and its copy number in Sulfolobus was increased by exposure of the cells to mitomycin C.