IMMUNOLOGICAL PROPERTIES OF A TYPE-C RETROVIRUS ISOLATED FROM CULTURED HUMAN T-LYMPHOMA CELLS AND COMPARISON TO OTHER MAMMALIAN RETROVIRUSES
IMMUNOLOGICAL PROPERTIES OF A TYPE-C RETROVIRUS ISOLATED FROM CULTURED HUMAN T-LYMPHOMA CELLS AND COMPARISON TO OTHER MAMMALIAN RETROVIRUSES
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DOI：
10.1128/jvi.38.3.906-915.1981
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发表时间：
1981-01-01
影响因子：
5.4
通讯作者：
GALLO, RC
中科院分区：
文献类型：
--
作者：
KALYANARAMAN, VS;SARNGADHARAN, MG;GALLO, RC
HTLV CR株（HTLVCR）是从人T细胞淋巴瘤细胞系分离的逆转录病毒。从该病毒中纯化出分子量为24，000的蛋白质p24。一些结果表明，该p24是HTLVCR的内部核心蛋白。p24与病毒核心共纯化。在破坏病毒后，它被标记有125 I，但当未破坏的病毒被碘化时，它就没有被标记。p24的量与HTLVCR的量成正比。在色谱特性方面，HTLVCR p24的行为与其他逆转录病毒的主要结构蛋白（24，000 - 30，000-MW蛋白）相似。兔抗血清提出的破坏HTLVCR沉淀标记的p24;沉淀的竞争由未标记的HTLVCR和细胞质蛋白从细胞生产HTLVCR，但不是由蛋白质从正常的人类细胞，包括正常生长的人类T细胞和几个培养的人类皮肤T细胞淋巴瘤系。来自几种哺乳动物B型[鼠乳腺肿瘤病毒]、C型[Rauscher鼠白血病、猿猴肉瘤、狒狒内源性、猫白血病、猫头鹰猴、鹿肾和牛白血病病毒]和D型[Mason-Pfizer猴病毒和松鼠猴逆转录病毒]病毒的蛋白质也未能在该沉淀中竞争。HTLVCR在几种哺乳动物C型和D型病毒的p30抗原的同源和种间测定中没有反应。这些观察结果与HTLVCR和其他逆转录病毒的逆转录酶之间的免疫学比较和核酸序列同源性研究一致，这些研究表明，各种HTLVCR分离株代表了在一些人类T细胞肿瘤中发现的新逆转录病毒。
HTLV strain CR (HTLVCR) is a retrovirus which was isolated from a human T-cell lymphoma cell line. A protein of MW 24,000, p24, was purified from this virus. Several results indicate that this p24 is an internal core protein of HTLVCR. The p24 copurified with viral cores. It was labeled with 125I after disruption of the virus but not when undisrupted virus was iodinated. The amount of p24 was directly proportional to the amount of HTLVCR. In chromatographic properties, the HTLVCR p24 behaved similarly to the major structural protein (24,000- to 30,000-MW protein) of other retroviruses. A rabbit antiserum raised against disrupted HTLVCR precipitated the labeled p24; the precipitation was competed for by unlabeled HTLVCR and by cytoplasmic proteins from cells producing HTLVCR but not by proteins from normal human cells, including normal growing human T-cells and several cultured human cutaneous T-cell lymphoma lines. Proteins from several mammalian type B [murine mammary tumor virus], type C [Rauscher murine leukemia, simian sarcoma, baboon endogenous, feline leukemia, owl monkey, deer kidney and bovine leukemia viruses] and type D [Mason-Pfizer monkey virus and squirrel monkey retrovirus] viruses also failed to compete in this precipitation. HTLVCR did not react in homologous and interspecies assays for p30 antigens of several mammalian type C and type D viruses. These observations agree with immunological comparisons between reverse transcriptase of HTLVCR and other retroviruses and nucleic acid sequence homology studies which indicate that the various HTLVCR isolates represent new retroviruses found in some human T-cell neoplasias.