Impairment of FtsHi5 Function Affects Cellular Redox Balance and Photorespiratory Metabolism in Arabidopsis.
Impairment of FtsHi5 Function Affects Cellular Redox Balance and Photorespiratory Metabolism in Arabidopsis.
复制标题
FtsHi5 功能受损会影响拟南芥细胞氧化还原平衡和光呼吸代谢。
DOI：
10.1093/pcp/pcy174
复制
发表时间：
2018
影响因子：
4.9
通讯作者：
Zhu Guohui
中科院分区：
文献类型：
--
作者：
Wang Ting;Li Sihui;Chen Dan;Xi Yue;Xu Xuezhong;Ye Nenghui;Zhang Jianhua;Peng Xinxiang;Zhu Guohui
光呼吸是植物在有氧条件下进行光合作用、发育和生长的重要过程。近年来的研究表明，光呼吸是一个与植物初级代谢网络和细胞内氧化还原系统相结合的开放系统，但调节光呼吸的机制尚不清楚。通过前向遗传方法，我们鉴定了一个光呼吸突变体pr1(photorespiratoryrelated1)，该突变体在环境空气中产生叶绿素含量降低，甘氨酸和丝氨酸积累减少的叶绿素和较小的光呼吸生长表型。在二氧化碳浓度升高的条件下，植物的形态和生理缺陷在很大程度上可以消除。遗传定位和互补证实PR1编码FtsH（丝化温度敏感H）样蛋白FtsHi5。在dex诱导的RNAi转基因植株中，ftshi5表达减少产生了与pr1相似的生长表型（ftsHi5-1）。转录组分析显示氧化还原相关基因表达模式发生改变，衰老相关基因表达增加。结合观察到FtsHi5突变体中光系统II (PSII) D1和D2蛋白的积累减少以及活性氧（ROS）的过度积累，我们假设FtsHi5在维持细胞氧化还原平衡中起作用，从而调节光呼吸代谢。
Photorespiration is an essential process for plant photosynthesis, development and growth in aerobic conditions. Recent studies have shown that photorespiration is an open system integrated with the plant primary metabolism network and intracellular redox systems, though the mechanisms of regulating photorespiration are far from clear. Through a forward genetic method, we identified a photorespiratory mutantpr1(photorespiratoryrelated1), which produced a chlorotic and smaller photorespiratory growth phenotype with decreased chlorophyll content and accumulation of glycine and serine in ambient air. Morphological and physiological defects inpr1plants can be largely abolished under elevated CO2conditions. Genetic mapping and complementation confirmed that PR1 encodes an FtsH (Filamentation temperature-sensitive H)-like protein, FtsHi5. ReducedFtsHi5expression in DEX-induced RNAi transgenic plants produced a similar growth phenotype withpr1(ftsHi5-1). Transcriptome analysis suggested a changed expression pattern of redox-related genes and an increased expression of senescence-related genes inDEX: RNAi-FtsHi5seedlings. Together with the observation that decreased accumulation of D1 and D2 proteins of photosystem II (PSII) and over-accumulation of reactive oxygen species (ROS) inftsHi5mutants, we hypothesize that FtsHi5 functions in maintaining the cellular redox balance and thus regulates photorespiratory metabolism.