Sustained proliferation accompanies distraction osteogenesis in the rat
Sustained proliferation accompanies distraction osteogenesis in the rat
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DOI：
10.1002/jor.1100150412
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发表时间：
1997-07-01
影响因子：
2.8
通讯作者：
Lumpkin, CK
中科院分区：
文献类型：
--
作者：
Aronson, J;Shen, XC;Lumpkin, CK
这些研究的目的是比较牵张成骨过程中和非牵张骨折愈合过程中大鼠胫骨局部细胞增殖的模式。分别在术后2、10、20天对牵引成骨组和非牵引骨折组的骨标本进行分析。通过[H-3]胸苷代谢标记和增殖细胞核抗原抗体免疫细胞化学测定增殖。使用视频显微镜对指定区域内的阳性染色细胞进行计数。掺入[H-3]胸苷的细胞数量与交替连续载玻片上增殖细胞核抗原阳性细胞数量正相关（r（2）=0.78）。在第2天，后一种细胞在两组中主要局限于骨髓和骨膜，并且每平方毫米的细胞数也相等。在第10和20天，增殖细胞核抗原阳性细胞在牵张成骨组的近端和远端初级基质前区均占优势。而非牵张骨折组的增殖细胞核抗原阳性细胞散在分布于整个愈合区。这些细胞中有更多的是在初级基质前区，而不是在任何位置内的非分散性骨折愈合区。在两组中，邻近手术区域的骨髓中这些细胞的数量从第2天到第10天下降。结果表明：（a）增殖细胞核抗原免疫染色是细胞循环的可靠指标;（B）到第10天，牵张成骨的特征在于增殖细胞的区域特异性模式;（c）牵张成骨抑制骨折后差距内增殖的刺激。
These studies were conducted to compare the local cellular proliferation patterns in the rat tibia during distraction osteogenesis with those during nondistracted fracture healing. Bone specimens from distraction osteogenesis and nondistracted fracture groups were analyzed 2, 10, and 20 days after surgery. Proliferation was determined by metabolic labeling with [H-3]thymidine and by immunocytochemistry with an antibody to proliferating cell nuclear antigen. Videomicroscopy was used to count the cells staining positively within specified regions. The number of cells incorporating [H-3]thymidine was positively correlated (r(2)=0.78) with the number of proliferating cell nuclear antigen positive cells on alternating serial slides. At day 2, the latter cells were largely confined to the bone marrow and periosteum in both groups, and the cell numbers per mm(2) were also equivalent. At days 10 and 20, the proliferating cell nuclear antigen positive cells predominated in both the proximal and distal primary matrix front zones in the distraction osteogenesis group. Tn contrast, the proliferating cell nuclear antigen positive cells in the nondistracted fracture group were scattered throughout the healing area. Significantly more of these cells were in the primary matrix front zones than in any location within the nondistracted fracture-healing area. The number of these cells in the bone marrow adjacent to the surgical area declined from day 2 to day 10 in both groups. The results suggest that (a) proliferating cell nuclear antigen immunostaining is a reliable indicator of cycling cells; (b) by day 10, distraction osteogenesis is characterized by a zone specific pattern of proliferating cells; and (c) distraction osteogenesis prolongs the stimulation of proliferation within the gap after fracture.