Electrodes modified with lipid membranes to study quinone oxidoreductases.
Electrodes modified with lipid membranes to study quinone oxidoreductases.
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DOI：
10.1042/bst0370707
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发表时间：
2009-08
影响因子：
3.9
通讯作者：
Jeuken LJ
中科院分区：
文献类型：
--
作者：
Weiss SA;Jeuken LJ
对苯二酚氧化还原酶是一类催化氧化或还原结合在膜上的对苯二酚/对苯二酚的膜酶。由于酶及其底物的疏水性，这些酶对苯二酚/对苯二酚的转化很难研究。我们描述了对苯二酚和对苯二酚氧化还原酶的一些生化性质，然后更详细地研究了两种模型膜，它们可以用来研究具有天然脂亲性对苯二酚底物的天然膜环境中的对苯二酚氧化还原酶。将用这些模型膜获得的结果与使用水溶性苯醌类似物的经典酶分析进行了比较。
Quinone oxidoreductases are a class of membrane enzymes that catalyse the oxidation or reduction of membrane-bound quinols/quinones. The conversion of quinone/quinol by these enzymes is difficult to study due to the hydrophobic nature of the enzymes and their substrates. We describe some biochemical properties of quinones and quinone oxidoreductases and then look in more detail at two model membranes that can be used to study quinone oxidoreductases in a native-like membrane environment with their native lipophylic quinone substrates. The results obtained with these model membranes are compared to classical enzyme assays that use water-soluble quinone analogues.