Isolation of a Novel Coronavirus from a Man with Pneumonia in Saudi Arabia
Isolation of a Novel Coronavirus from a Man with Pneumonia in Saudi Arabia
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DOI：
10.1056/nejmoa1211721
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发表时间：
2012-11-08
影响因子：
158.5
通讯作者：
Fouchier, Ron A. M.
中科院分区：
文献类型：
--
作者：
Zaki, Ali Moh;van Boheemen, Sander;Fouchier, Ron A. M.
从沙特阿拉伯一名60岁男子的痰中分离出一种以前未知的冠状病毒，该男子出现急性肺炎和随后的肾衰竭，并导致死亡。该病毒（称为HCoV-EMC）在细胞培养中容易复制，产生变圆、分离和合胞体形成的细胞病变效应。该病毒代表一种新型β冠状病毒。已知的最接近的亲属是蝙蝠冠状病毒HKU 4和HKU 5。在这里，临床数据，病毒分离，和分子鉴定。临床表现与2003年严重急性呼吸道综合征（SARS）爆发的情况非常相似，并提醒我们动物冠状病毒可在人类中引起严重疾病。
A previously unknown coronavirus was isolated from the sputum of a 60-year-old man who presented with acute pneumonia and subsequent renal failure with a fatal outcome in Saudi Arabia. The virus (called HCoV-EMC) replicated readily in cell culture, producing cytopathic effects of rounding, detachment, and syncytium formation. The virus represents a novel betacoronavirus species. The closest known relatives are bat coronaviruses HKU4 and HKU5. Here, the clinical data, virus isolation, and molecular identification are presented. The clinical picture was remarkably similar to that of the severe acute respiratory syndrome (SARS) outbreak in 2003 and reminds us that animal coronaviruses can cause severe disease in humans.