Subgroup J avian leukosis virus infection of chicken dendritic cells induces apoptosis via the aberrant expression of microRNAs.
Subgroup J avian leukosis virus infection of chicken dendritic cells induces apoptosis via the aberrant expression of microRNAs.
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DOI：
10.1038/srep20188
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发表时间：
2016-02-01
影响因子：
4.6
通讯作者：
Liao M
中科院分区：
文献类型：
--
作者：
Liu D;Dai M;Zhang X;Cao W;Liao M
J亚型禽白血病病毒（ALV-J）是一种致癌逆转录病毒，可引起免疫抑制并增加继发感染的易感性。先天免疫系统是预防细菌和病毒感染的第一道防线，树突状细胞（dc）在先天免疫中起着重要作用。由于骨髓是一种易受ALV-J感染的器官，该病毒可能影响骨髓源性dc的产生。本研究采用体外培养的树突状细胞研究ALV感染对树突状细胞的影响。结果表明，ALV-J可以在分化早期感染这些细胞，ALV-J感染dc后导致细胞凋亡。未感染和感染dc的miRNA测序数据显示122个差异表达的miRNA，其中115个在ALV-J感染后表达上调，另外7个表达显著下调。表现出最高水平上调的mirna可能抑制营养加工和代谢功能。这些结果表明ALV-J感染可通过microRNA的异常表达诱导鸡dc细胞凋亡。这些结果为进一步研究表观遗传对alv - j诱导的免疫抑制的影响奠定了坚实的基础。
Subgroup J avian leukosis virus (ALV-J) is an oncogenic retrovirus that causes immunosuppression and enhances susceptibility to secondary infection. The innate immune system is the first line of defense in preventing bacterial and viral infections, and dendritic cells (DCs) play important roles in innate immunity. Because bone marrow is an organ that is susceptible to ALV-J, the virus may influence the generation of bone marrow-derived DCs. In this study, DCs cultured in vitro were used to investigate the effects of ALV infection. The results revealed that ALV-J could infect these cells during the early stages of differentiation, and infection of DCs with ALV-J resulted in apoptosis. miRNA sequencing data of uninfected and infected DCs revealed 122 differentially expressed miRNAs, with 115 demonstrating upregulation after ALV-J infection and the other 7 showing significant downregulation. The miRNAs that exhibited the highest levels of upregulation may suppress nutrient processing and metabolic function. These results indicated that ALV-J infection of chicken DCs could induce apoptosis via aberrant microRNA expression. These results provide a solid foundation for the further study of epigenetic influences on ALV-J-induced immunosuppression.