Mutation of AREA affects growth, sporulation, nitrogen regulation, and pathogenicity in Colletotrichum gloeosporioides
Mutation of AREA affects growth, sporulation, nitrogen regulation, and pathogenicity in Colletotrichum gloeosporioides
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DOI：
10.1016/j.fgb.2016.12.006
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发表时间：
2017-02-01
影响因子：
3
通讯作者：
Prusky, Dov
中科院分区：
文献类型：
--
作者：
Bi, Fangcheng;Ment, Dana;Prusky, Dov
加塔转录因子AreA是一种全局氮调节因子，在微生物中存在良好氮源的情况下，其限制复杂和不良氮源的利用。在这项研究中，我们报告了一个AreA同源基因的生物学功能的水果采后病原体炭疽gloeosporioides（CSARA基因）。靶向基因缺失突变体areA表现出显着减少营养生长，分生孢子产量增加，孢子萌发率略有下降。定量RT-PCR（gRT-PCR）分析表明，在氮限制条件下，AreA的表达被高度诱导。此外，与野生型和互补菌株相比，Delta areA突变菌株中的氮代谢相关基因被错误调节。致病性测定表明，Delta areA突变株的致病力受果实寄主的氮含量的影响，而不是碳含量。综上所述，我们的研究结果表明，在炭疽菌的真菌发育，分生孢子的生产，氮代谢和毒力的调节中起着至关重要的作用。(C)2017爱思唯尔公司All rights reserved.
The GATA transcription factor AreA is a global nitrogen regulator that restricts the utilization of complex and poor nitrogen sources in the presence of good nitrogen sources in microorganisms. In this study, we report the biological function of an AreA homolog (the CgareA gene) in the fruit postharvest pathogen Colletotrichum gloeosporioides. Targeted gene deletion mutants of areA exhibited significant reductions in vegetative growth, increases in conidia production, and slight decreases in conidial germination rates. Quantitative RT-PCR (gRT-PCR) analysis revealed that the expression of AreA was highly induced under nitrogen-limiting conditions. Moreover, compared to wild-type and complemented strains, nitrogen metabolism-related genes were misregulated in Delta areA mutant strains. Pathogenicity assays indicated that the virulence of Delta areA mutant strains were affected by the nitrogen content, but not the carbon content, of fruit hosts. Taken together, our results indicate that CgareA plays a critical role in fungal development, conidia production, regulation of nitrogen metabolism and virulence in Colletotrichum gloeosporioides. (C) 2017 Elsevier Inc. All rights reserved.