Close phenotypic and functional similarities between human and murine alphabeta T cells expressing invariant TCR alpha-chains.
Close phenotypic and functional similarities between human and murine alphabeta T cells expressing invariant TCR alpha-chains.
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表达不变 TCR α 链的人和鼠 Alphata T 细胞的表型和功能非常相似。
DOI：
10.4049/jimmunol.158.12.5603
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发表时间：
1997
影响因子：
4.4
通讯作者：
M. Bonneville
中科院分区：
文献类型：
--
作者：
F. Davodeau;M. Peyrat;A. Necker;R. Dominici;Frédéric Blanchard;C. Leget;Joëlle Gaschet;P. Costa;Yannick Jacques;A. Godard;Henri Vié;Alessandro Poggi;F. Romagné;M. Bonneville
几项研究已经证明存在表达具有高度保守的不变连接序列的V α 14 + TCR α链的鼠NK 1.1 + α T细胞亚群，并且当暴露于CD 1+刺激细胞时能够分泌Th 2细胞因子。在人类中，最近已经描述了携带与由鼠NK 1.1细胞表达的那些高度同源的不变V α 24 + TCR α链的α T细胞。在这里，我们表明，这些细胞（称为V α 24 inv T细胞）和小鼠NK 1.1 + α T细胞在几个方面彼此相似。首先，与它们的鼠对应物一样，表达高水平V α 24 inv TCR的T细胞可以是CD 4-CD 8-双阴性（DN）或CD 4+，但它们从不表达异二聚体CD 8分子。第二，大多数V α 24 inv T细胞被NKRP 1特异性mAb明亮染色，但不被针对NK复合物的其他II型跨膜蛋白的mAb染色。第三，DN和特别是CD 4 + V α 24 inv T细胞极大地富集IL-4生产者。伴随表达的高度保守的TCR的一组特定的NK标志物和Th 2细胞因子在人类和小鼠α T细胞表明这些表型和功能特性的协调收购。此外，人V α 24 inv T细胞的相对高的频率（目前显示其平均代表1/500 PBL）和该细胞亚群在生理条件下的大小的高度个体间变化导致携带不变TCR的α T细胞在大量免疫应答中发挥主要作用。
Several studies have demonstrated the existence of a murine NK1.1+ alphabeta T cell subset expressing V alpha14+ TCR alpha-chains with highly conserved invariant junctional sequences and able to secrete Th2 cytokines when exposed to CD1+ stimulator cells. In humans, alphabeta T cells carrying invariant V alpha24+ TCR alpha-chains highly homologous to those expressed by murine NK1.1 cells have been recently described. Here we show that these cells (referred to as V alpha24inv T cells) and murine NK1.1+ alphabeta T cells resemble each other in several ways. First, like their murine counterparts, T cells expressing high levels of V alpha24inv TCRs can be either CD4- CD8- double negative (DN) or CD4+, but they never express heterodimeric CD8 molecules. Second, most V alpha24inv T cells are brightly stained by NKRP1-specific mAb but not by mAb directed against other type II transmembrane proteins of the NK complex. Third, DN and particularly CD4+ V alpha24inv T cells are greatly enriched for IL-4 producers. The concomitant expression of highly conserved TCRs of a particular set of NK markers and of Th2 cytokines in human and murine alphabeta T cells suggests a coordinate acquisition of these phenotypic and functional properties. Furthermore, the relatively high frequency of human V alpha24inv T cells, which are presently shown to represent on average 1/500 PBL, and the high interindividual variations of the size of this cell subset under physiologic conditions go for a major role played by alphabeta T cells carrying invariant TCR in a large array of immune responses.