Quantitative determination of 26 steroids in eggs from various species using liquid chromatography-triple quadrupole-mass spectrometry
Quantitative determination of 26 steroids in eggs from various species using liquid chromatography-triple quadrupole-mass spectrometry
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液相色谱-三重四极杆质谱法定量测定不同物种鸡蛋中的26种类固醇
DOI：
10.1016/j.chroma.2014.05.084
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发表时间：
2014-08-22
影响因子：
4.1
通讯作者：
Chen, Gang
中科院分区：
文献类型：
--
作者：
Mi, Xiaoxia;Li, Sicong;Chen, Gang
开发了一种分析蛋类基质中26种类固醇的方法。该方法采用液相色谱 - 串联质谱（LC - MS/MS），在电喷雾电离模式（ESI）下进行。操作步骤包括用乙腈提取，用氯化锌（ZnCl₂）去除磷脂，然后用Plexa柱进行固相萃取（SPE）净化。使用柱后灌注法直接观察了ZnCl₂对磷脂去除的效果。采用浅梯度法优化了SPE的洗涤和洗脱条件。使用酶水解法测定游离和结合形式的类固醇。所开发的方法具有令人满意的精密度（相对标准偏差（RSD）……（此处原文未完整给出RSD相关内容）
A method for analyzing 26 types of steroids in egg matrix was developed. The method used liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in electrospray ionization mode (ESI). The procedure involved extraction with acetonitrile and removal of phospholipids by zinc chloride (ZnCl2) followed by SPE cleanup with a Plexa cartridge. The effect of ZnCl2 on phospholipid removal was directly observed using the post column infusion procedure. The SPE washing and elution conditions were optimized using a shallow gradient procedure. The free and conjugated steroids forms were determined using enzyme hydrolysis. The developed method resulted in satisfactory precision (RSD