Retarded diffusion and confinement of membrane-bound molecules in a patterned hybrid membrane of phospholipid bilayers and monolayers
Retarded diffusion and confinement of membrane-bound molecules in a patterned hybrid membrane of phospholipid bilayers and monolayers
复制标题
磷脂双层和单层图案化杂化膜中膜结合分子的延迟扩散和限制
DOI：
10.1021/acs.jpcb.2c06053
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发表时间：
2023
期刊：
影响因子：
3.3
通讯作者：
K.
中科院分区：
文献类型：
--
作者：
Tanimoto;Y.;Yoshimura;Y.;Hayashi;F.;Morigaki;K.
生物膜是一种复杂的二维流体，其中各种分子相互作用调节膜相关分子的横向扩散。膜外蛋白固定膜蛋白或脂质会阻碍扩散。此外，磷脂双分子层内两个单层小叶之间通过交叉作用的耦合也起着重要的作用，尽管这种作用仍然难以捉摸。在这里，我们制作了一个具有图案双层/单层区域的底物支持的模型膜，以探索叶间耦合的影响。利用光刻技术制备了一种聚二乙炔磷脂的图图化单层，即1,2-二（10,12-三osadiynoyl）-sn-甘油-3-磷酸胆碱（DiynePC），并用于形成图图化脂质双层和单层。在聚合的单分子层上形成磷脂单分子层。双层/单层杂交膜是连续流动的，但单层区域的横向扩散明显受阻，提示叶间耦合的影响。我们重建了视紫红质（Rh）和g蛋白转导蛋白（Gt）作为模型跨膜蛋白和外周蛋白。Rh仅在双分子层区域横向扩散，而gt在双分子层和单分子层区域均扩散。图案化的双层/单层杂交膜以可控的方式再现了膜结合分子的延迟扩散和限制，并提供了对细胞膜中半透围栏的物理化学和功能作用的见解。
The biological membrane is a complex two-dimensional fluid, in which various molecular interactions regulate the lateral diffusion of membrane-associated molecules. Pinning of membrane proteins or lipids by extra-membrane proteins impedes the diffusion. In addition, coupling between two monolayer leaflets within a phospholipid bilayer via interdigitation plays important roles, though this effect remains elusive. Here, we fabricate a substrate-supported model membrane with patterned bilayer/monolayer regions to explore the influences of interleaflet coupling. A patterned monolayer of polymerized diacetylene phospholipid, 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (DiynePC), was lithographically generated and used to form patterned lipid bilayers and monolayers. A phospholipid monolayer was formed on top of the polymerized monolayer. The bilayer/monolayer hybrid membrane was continuous and fluid, but lateral diffusion in the monolayer region was significantly retarded, suggesting the influences of interleaflet coupling. We reconstituted photoreceptor rhodopsin (Rh) and G-protein transducin (Gt) as model transmembrane and peripheral proteins. Rh diffused laterally only in the bilayer region, whereas Gtdiffused in both bilayer and monolayer regions. The patterned hybrid bilayer/monolayer membrane reproduces the retarded diffusion and confinement of membrane-bound molecules in a controlled manner and provides insight into the physicochemical and functional roles of semipermeable corrals in the cell membrane.