Allogenous Tendon Stem/Progenitor Cells in Silk Scaffold for Functional Shoulder Repair
Allogenous Tendon Stem/Progenitor Cells in Silk Scaffold for Functional Shoulder Repair
复制标题
丝支架中的同种异体肌腱干/祖细胞用于功能性肩部修复
DOI：
10.3727/096368911x627453
复制
发表时间：
2012-01-01
影响因子：
3.3
通讯作者：
Ouyang, Hong-Wei
中科院分区：
文献类型：
--
作者：
Shen, Weiliang;Chen, Jialin;Ouyang, Hong-Wei
肌腱干/祖细胞（TSPC）最近被发现在肌腱组织。本研究的目的是探讨TSPC种植的针织丝胶原海绵支架用于功能性肩关节修复。在体外研究了TSPCs的多分化潜能、增殖和免疫特性，而在兔模型中在体内评价了TSPC接种的针织丝胶原海绵支架在促进肩袖再生中的功效。TSPC表现出通用干细胞特征（即，克隆形成性、高增殖能力和多分化潜能）、非免疫原性和免疫抑制，在我们的支架上体外增殖良好。在兔肩袖损伤模型中植入同种异体TSPC接种的支架没有引起免疫反应，而是在术后4周和8周增加了成纤维细胞向内生长并减少了植入部位内的淋巴细胞浸润。12周后，与对照组相比，同种异体TSPC治疗组表现出增加的胶原沉积，并具有更好的结构和生物力学性能。因此，本研究表明，同种异体TSPC接种的针织丝胶原海绵支架通过分化成腱细胞和分泌防止免疫排斥的抗炎细胞因子来增强肩袖肌腱再生的功效。因此，同种异体TSPC接种的针织丝胶原海绵支架可以是肌腱组织工程的临床有用的应用。
Tendon stem/progenitor cells (TSPCs) were recently identified within tendon tissues. The aim of this study was to investigate TSPC-seeded knitted silk collagen sponge scaffold for functional shoulder repair. The multidifferentiation potential, proliferation, and immune properties of TSPCs were investigated in vitro, while the efficacy of TSPC-seeded knitted silk collagen sponge scaffolds in promoting rotator cuff regeneration was evaluated in vivo within a rabbit model. TSPCs, which exhibited universal stem cell characteristics (i.e., clonogenicity, high proliferative capacity, and multidifferentiation potential), nonimmunogenicity, and immunosuppression, proliferated well on our scaffold in vitro. Implantation of allogenous TSPC-seeded scaffolds within a rabbit rotator cuff injury model did not elicit an immunological reaction, but instead increased fibroblastic cell ingrowth and reduced infiltration of lymphocytes within the implantation sites at 4 and 8 weeks postsurgery. After 12 weeks, the allogenous TSPC-treated group exhibited increased collagen deposition and had better structural and biomechanical properties compared to the control group. This study thus demonstrated that the allogenous TSPC-seeded knitted silk collagen sponge scaffold enhanced the efficacy of rotator cuff tendon regeneration by differentiating into tenocytes, and by secreting anti-inflammatory cytolcines that prevent immunological rejection. Hence, allogenous TSPC-seeded knitted silk collagen sponge scaffolds can be a clinically useful application for tendon tissue engineering.