Pilot-scale production of carboxymethylcellulase from rice hull by Bacillus amyloliquefaciens DL-3
Pilot-scale production of carboxymethylcellulase from rice hull by Bacillus amyloliquefaciens DL-3
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DOI：
10.1007/s12257-007-0149-y
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发表时间：
2008-03-01
影响因子：
3.2
通讯作者：
Lee, Jin-Woo
中科院分区：
文献类型：
--
作者：
Jo, Kang-Ik;Lee, You-Jung;Lee, Jin-Woo
研究了解淀粉芽孢杆菌DL-3产羧甲基纤维素酶（CMCase）的中试条件。B产羧甲基纤维素酶的最佳碳源和氮源。解淀粉菌DL-3的最适培养基为稻壳船体和蛋白胨，其最适浓度分别为5.0%和0.20%（w/v）。CMCase的最适生产温度和初始pH值分别为37 ℃和6.8.在7 L的生物反应器中，最佳搅拌速度和通气量分别为300 rpm和1.0 vvm，这与B细胞生长的最佳搅拌速度和通气量不同。解淀粉菌DL-3。B的CMCase产量最高。在7 L和100 L生物反应器中，以5.0%（w/v）的水稻船体为碳源，对解淀粉菌DL-3的最适产酶量分别为220和367 U/mL。(C)KSBB
Optimal conditions for pilot-scale production of the carboxymethylcellulase (CMCase) by Bacillus amyloliquefaciens DL-3 were investigated. The best carbon and nitrogen sources for the production of CMCase by B. amyloliquefaciens DL-3 were found to be rice hull and peptone and their optimal concentrations were 5.0 and 0.20% (w/v), respectively. Optimal temperature and initial pH for the production of CMCase were 37 C and 6.8. Optimal agitation speed and aeration rate for the production of CMCase were 300 rpm and 1.0 vvm in a 7 L bioreactor, which were different from those for the cell growth of B. amyloliquefaciens DL-3. The highest productions of CMCase by B. amyloliquefaciens DL-3 from 5.0% (w/v) rice hull as a carbon source under optimal conditions in a 7 or 100 L bioreactor were 220 and 367 U/mL, respectively. (C) KSBB.