Application of the quartz crystal microbalance to the monitoring of Staphylococcus epidermidis antigen-antibody agglutination.
Application of the quartz crystal microbalance to the monitoring of Staphylococcus epidermidis antigen-antibody agglutination.
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DOI：
10.1016/s0731-7085(99)00026-6
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发表时间：
1999-06
影响因子：
3.4
通讯作者：
K. Pavey;Z. Ali;C. Olliff;F. Paul
中科院分区：
文献类型：
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作者：
K. Pavey;Z. Ali;C. Olliff;F. Paul
由于抗原与其特异性抗体的凝集而引起的溶液性质的变化先前已被用作感染的标志物。该方法已被修改，以允许使用石英晶体微量天平（QCM）的频率响应遵循物种之间的结合活性。表皮葡萄球菌的Bayston凝集板测定已被修改，允许QCM的电极作为凝集发生时溶液性质变化的直接传感器。将抗体和抗原引入到晶体表面，并且随着晶体谐振频率的变化，凝集过程被跟踪。已知感染该微生物的血清的滴度为3.9×10−2% v/v（−118 Hz，±12 SD，N=9），与三次平板试验的滴度相匹配。未感染血清在该浓度下没有频率变化，产生的滴度为2.5×10−2% v/v，再次与平板滴度匹配（N=3）。感染的血清比未感染的血清反应快40倍。压电石英晶体法在小于15 min内给出阳性或阴性诊断，而平板法需要24 h。
The change in solution properties due to the agglutination of an antigen with its specific antibody has previously been used as a marker of infection. This method has been modified to allow the binding activity between species to be followed using the frequency response of a quartz crystal microbalance (QCM). The Bayston agglutination plate assay for Staphylococcus epidermidis has been modified to allow the electrode of a QCM to act as a direct sensor for the change in solution properties as agglutination occurs. Antibody and antigen were introduced to the crystal surface and the agglutination process was followed as a change in crystal resonant frequency. Serum, known to be infected with the organism, gave a titre of 3.9×10−2% v/v (−118 Hz, ±12 SD, N=9) matching that given by triplicate plate assay. Uninfected serum gave no frequency changes at this concentration, yielding a titre of 2.5×10−2% v/v again matching the plate titre (N=3). Infected serum gave responses 40 times faster then those of the uninfected serum. The piezoelectric quartz crystal method gave a positive or negative diagnosis in <15 min compared with the 24 h required for the plate assay.