The Campylobacter jejuni NCTC11168 capsule prevents excessive cytokine production by dendritic cells.
The Campylobacter jejuni NCTC11168 capsule prevents excessive cytokine production by dendritic cells.
复制标题
空肠弯曲杆菌 NCTC11168 胶囊可防止树突状细胞产生过多的细胞因子。
DOI：
10.1007/s00430-011-0214-1
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发表时间：
2012
影响因子：
5.4
通讯作者：
Rose A
中科院分区：
文献类型：
--
作者：
Rose A
Campylobacter jejuniis the leading cause of human bacterial gastroenteritis worldwide, mainly caused by handling and consumption of contaminated poultry. However, the immune response to infection is poorly understood. Here, the impact of theC. jejunicapsule, flagella and theN-linked glycosylation system on cytokine production by dendritic cells was investigated. Bone marrow-derived murine dendritic cells (BMDCs) infected withC. jejunilacking theN-linked glycosylation system produced similar amounts of cytokines compared to cells infected withC. jejuni11168H wild-type (WT) cultures.C. jejuniflagellin FlaA mutants elicited reduced IL-6 and IL-10 production in BMDCs compared toC. jejuniWT and this reduction was more pronounced in TLR4−/−BMDCs. An acapsularC. jejunimutant as well as a mutant lacking theO-methyl phosphoramidate modification of the capsule elicited a higher cytokine response in BMDCs. Experiments with TLR4−/−BMDCs revealed that this increased cytokine production was not solely dependent on signalling through TLR4. Therefore, theC. jejunicapsule is important to prevent excessive cytokine production by BMDCs and even minor changes in capsule composition such as the lack of theO-methyl phosphoramidate modification can lead to increased cytokine production.
