Disappearance of a major cell‐type specific surface glycoprotein antigen (SF) after transformation of fibroblasts by rous sarcoma virus
Disappearance of a major cell‐type specific surface glycoprotein antigen (SF) after transformation of fibroblasts by rous sarcoma virus
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劳斯肉瘤病毒转化成纤维细胞后，主要细胞类型特异性表面糖蛋白抗原（SF）消失
DOI：
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发表时间：
1974
影响因子：
6.4
通讯作者：
E. Ruoslahti
中科院分区：
文献类型：
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作者：
A. Vaheri;E. Ruoslahti
已知正常鸡成纤维细胞含有组织特异性表面组分，这是一种主要的糖蛋白（SF）抗原，也存在于鸡血清中。这种抗原在由五种不同的劳斯肉瘤病毒株转化的成纤维细胞中的量大大减少或不存在。当维持在非允许温度下时，用对转化温度敏感的病毒突变体感染的成纤维细胞恢复SF抗原。用非转化禽C型病毒进行生产性感染不会改变正常成纤维细胞特征性SF抗原的水平。聚丙烯酰胺凝胶电泳结果表明，总细胞提取物中具有表观分子量的蛋白质。重量145，000和mol。重量210，000在成纤维细胞转化后大大减少。合成145，000 mol.重量脉冲标记实验暗示了在转化时多肽丢失，其中正常的成纤维细胞将[35S]甲硫氨酸掺入上述多肽中，而转化的细胞失去了这种能力。这种多肽在正常细胞中存在而在转化细胞中不存在，这是其独特之处，似乎具有异常高的周转率。间接证据表明，145，000和210，000多肽是SF抗原的分子对应物。SF分子数量的转化相关变化支持我们的提议，即组织特异性表面糖蛋白（如SF抗原）在正常细胞膜上表达分化阶段，并可能参与细胞间生长和运动的控制。
Normal chick fibroblasts are known to contain a tissue‐specific surface component, a major glycoprotein (SF) antigen that is also present in chicken serum. This antigen was greatly reduced in amount or absent in fibroblasts transformed by five different Rous sarcoma virus strains. Fibroblasts infected with virus mutants temperature sensitive for transformation recovered the SF antigen when maintained at the non‐permissive temperature. Productive infection with a non‐transforming avian type‐C virus did not alter the level of SF antigen characteristic of normal fibroblasts. Polyacrylamide gel electrophoresis of total cell extracts indicated that proteins with apparent mol. wt. of 145,000 and mol. wt. 210,000 were greatly decreased following transformation of the fibroblasts. That the ability to synthetize the 145,000 mol. wt. polypeptide was lost upon transformation was implied by pulse labelling experiments, in which normal fibroblasts incorporate [35S] methionine into the above polypeptide whereas transformed cells lose this ability. This polypeptide, unique in its presence in normal but not in transformed cells, appears to have an exceptionally high rate of turnover. Indirect evidence suggests that the 145,000 and 210,000 polypeptides are molecular counterparts of the SF antigen. The transformation‐associated change in the quantity of the SF molecule supports our proposal that tissue‐specific surface glycoproteins, such as the SF antigen, express the stage of differentiation at the membrane of normal cells and may be involved in intercellular control of growth and movement.