CDNA SEQUENCE OF 2 DISTINCT PITUITARY PROTEINS HOMOLOGOUS TO KEX2 AND FURIN GENE-PRODUCTS - TISSUE-SPECIFIC MESSENGER-RNAS ENCODING CANDIDATES FOR PRO-HORMONE PROCESSING PROTEINASES
CDNA SEQUENCE OF 2 DISTINCT PITUITARY PROTEINS HOMOLOGOUS TO KEX2 AND FURIN GENE-PRODUCTS - TISSUE-SPECIFIC MESSENGER-RNAS ENCODING CANDIDATES FOR PRO-HORMONE PROCESSING PROTEINASES
复制标题
DOI：
10.1089/dna.1990.9.415
复制
发表时间：
1990-07-01
影响因子：
3.1
通讯作者：
CHRETIEN, M
中科院分区：
文献类型：
--
作者：
SEIDAH, NG;GASPAR, L;CHRETIEN, M
基于丝氨酸蛋白酶活性位点附近序列保守性的概念，聚合酶链反应应用于mRNA扩增，使我们能够获得一个260 bp的探针，用于筛选小鼠垂体cDNA文库。所用引物来源于人furin的Ser* 和Asn* 活性位点的cDNA序列。从多个阳性克隆中获得两个cDNA序列。它们编码两种相似但不同的结构（mPC1和mPC2），每种结构都与酵母Kex2和人弗林蛋白酶同源。原位杂交（mPC 1）和北方印迹（mPC 1和mPC 2），均与酵母Kex 2和人弗林蛋白酶同源。原位杂交（mPC 1）和北方印迹（mPC 1 = 3.0 kb和mPC 2 = 2.8和4.8 kb）证明了表达的组织和细胞特异性，仅在内分泌和神经内分泌细胞内。这些数据表明，MPC 1和MPC 2代表组织特异性激素原转化蛋白酶的主要候选人。
Based on the concept of sequence conservation around the active sites of serine proteinases, polymerase chain reaction applied to mRNA amplification allowed us to obtain a 260-bp probe which was used to screen a mouse pituitary cDNA library. The primers used derived from the cDNA sequence of active sites of Ser* and Asn* of human furin. Two cDNA sequences were obtained from a number of positive clones. These code for two similar but distinct structures (mPC1 and mPC2), each being homologous to yeast Kex2 and human furin. In situ hybridization (mPC1) and Northern blots (mPC1 and mPC2), each being homologous to yeast Kex2 and human furin. In situ hybridization (mPC1) and Northern blots (mPC1 = 3.0 kb and mPC2 = 2.8 and 4.8 kb) demonstrated tissue and cellular specificity of expression, only within endocrine and neuroendocrine cells. These data suggest that mPC1 and mPC2 represent prime candidates for tissue-specific pro-hormone converting proteinases.