Target Profiling of an Anticancer Drug Curcumin by an In Situ Chemical Proteomics Approach
Target Profiling of an Anticancer Drug Curcumin by an In Situ Chemical Proteomics Approach
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DOI：
10.1007/978-1-0716-0954-5_13
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发表时间：
2021-01-01
期刊：
影响因子：
--
通讯作者：
Wang, Jigang
中科院分区：
文献类型：
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作者：
Liu, Dan-dan;Zou, Chang;Wang, Jigang
Interdisciplinary chemical proteomics approaches have been widely applied to the identification of specific targets of bioactive small molecules or drugs. In this chapter, we describe the application of a cell-permeable activity-based curcumin probe (Cur-P) with an alkyne moiety to detect and identify specific binding targets of curcumin in HCT116 colon cancer cells. Through click chemistry, a fluorescent tag or a biotin tag is attached to the probe-modified curcumin targets for visualization or affinity purification followed by mass spectrometric identification. A quantitative proteomics approach of isobaric tags for relative and absolute quantification (iTRAQ)(TM) is applied to distinguish specific curcumin targets from nonspecific binding proteins.
