Understanding nonviral nucleic acid delivery with quantum dot-FRET nanosensors.
Understanding nonviral nucleic acid delivery with quantum dot-FRET nanosensors.
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DOI：
10.2217/nnm.12.28
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发表时间：
2012-04
期刊：
影响因子：
--
通讯作者：
Leong KW
中科院分区：
文献类型：
--
作者：
Grigsby CL;Ho YP;Leong KW
核酸的非病毒传递是一种潜在的安全和可行的治疗方式遗传和获得性疾病。然而，目前的系统对于广泛的临床转化来说效率太低。改进载体的合理设计取决于对有效细胞内递送的速率限制障碍的定量和机制理解。核酸与载体的分离是障碍之一，这可以通过Förster共振能量转移（FRET）来分析，这是一种用于检测荧光标记分子之间相互作用的机制。当应用于聚合物或脂基纳米复合物的分子组分时，FRET提供了其络合状态，摄取，释放和降解的信息。最近，将量子点作为能量供体的FRET系统的设计改善了信号稳定性，允许长时间的测量，提高了灵敏度，实现了直接检测和多路复用的潜力。量子点和FRET的结合通过对传递障碍的趋同表征为非病毒核酸传递机制提供了新的见解，并有可能加速改进载体的设计，以实现核酸治疗和基因医学的潜力。
Nonviral delivery of nucleic acids is a potentially safe and viable therapeutic modality for inherited and acquired diseases. However, current systems have proven too inefficient for widespread clinical translation. The rational design of improved carriers depends on a quantitative, mechanistic understanding of the rate-limiting barriers to efficient intracellular delivery. Separation of the nucleic acid from the carrier is one of the barriers, which may be analyzed by Förster resonance energy transfer (FRET), a mechanism used to detect interactions between fluorescently labeled molecules. When applied to the molecular components of polymer or lipid-based nanocomplexes, FRET provides information on their complexation status, uptake, release and degradation. Recently, the design of FRET systems incorporating quantum dots as energy donors has led to improved signal stability, allowing prolonged measurements, as well as increased sensitivity, enabling direct detection and the potential for multiplexing. The union of quantum dots and FRET is providing new insights into the mechanisms of nonviral nucleic acid delivery through convergent characterization of delivery barriers, and has the potential to accelerate the design of improved carriers to realize the potential of nucleic acid therapeutics and gene medicine.
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