RECOMBINANT OUTER SURFACE PROTEIN-A FROM BORRELIA-BURGDORFERI INDUCES ANTIBODIES PROTECTIVE AGAINST SPIROCHETAL INFECTION IN MICE
RECOMBINANT OUTER SURFACE PROTEIN-A FROM BORRELIA-BURGDORFERI INDUCES ANTIBODIES PROTECTIVE AGAINST SPIROCHETAL INFECTION IN MICE
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DOI：
10.1093/infdis/164.1.123
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发表时间：
1991-07-01
影响因子：
6.4
通讯作者：
WALLICH, R
中科院分区：
文献类型：
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作者：
SIMON, MM;SCHAIBLE, UE;WALLICH, R
伯氏疏螺旋体的外表面蛋白A（OspA）以其天然形式分离自菌株B17和B31，并且作为重组蛋白分离自菌株B17。对天然OspA（菌株107）的内部肽的氨基酸序列分析揭示了与从OspA基因推导的序列的同一性。用三种OspA结构中的任一种重复免疫C57 BL/6和C.B-17小鼠导致产生与天然和重组OspA两者反应的单特异性超免疫血清。在转移对天然OspA（菌株B31）或重组OspA（菌株B37）具有特异性但不与重组41-kDa鞭毛蛋白相关抗原反应的免疫血清后，严重联合免疫缺陷（SCID）小鼠完全免受菌株B37的感染。OspA和OspB的单克隆抗体，而不是非外表面螺旋体结构，如鞭毛蛋白，p20，p65和p70的发现赋予保护在SCID小鼠OspA（和可能的OspB）一个有前途的候选疫苗莱姆病。
The outer surface protein A (OspA) of Borrelia burgdorferi was isolated in its native form from strains ZS7 and B31 and as a recombinant protein from strain ZS7. Amino acid sequence analysis of internal peptides of native OspA (strain ZS7) revealed identity with the sequence deduced from the OspA gene. Repeated immunization of C57BL/6 and C.B-17 mice with any of the three OspA structures resulted in the generation of monospecific hyperimmune sera reactive with both native and recombinant OspA. Upon transfer of immune sera specific for either native OspA (strain B31) or recombinant OspA (strain ZS7) but not of those reactive with the recombinant 41-kDa flagellin-associated antigen, severe combined immunodeficient (SCID) mice were completely protected against infection with strain ZS7. The finding that monoclonal antibodies to OspA and to OspB but not to non-outer surface spirochetal structures such as flagellin, p20, p65, and p70 conferred protection in SCID mice makes OspA (and possibly OspB) a promising candidate vaccine against Lyme disease.