Synthesis of Selenium-Triphosphates (dNTPαSe) for More Specific DNA Polymerization
Synthesis of Selenium-Triphosphates (dNTPαSe) for More Specific DNA Polymerization
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合成三磷酸硒 (dNTP α Se) 以实现更特异性的 DNA 聚合
DOI：
10.1002/anie.201901113
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发表时间：
2019-06-03
影响因子：
16.6
通讯作者：
Huang, Zhen
中科院分区：
文献类型：
--
作者：
Hu, Bei;Wang, Yitao;Huang, Zhen
2 '-脱氧核苷5 '-（α-P-硒基）-三磷酸（dNTP α Se）已经使用无保护的一锅法策略方便地合成。每种dNTP α Se的两种非对映体之一可以被DNA聚合酶有效识别，而另一种既不是底物也不是抑制剂。此外，这种Se原子修饰可以显著抑制由错误引发引起的非特异性DNA聚合。通过聚合酶链反应用dNTP α Se扩增的Se-DNA具有与相应的天然DNA相同的序列。总之，通过用dNTP α Se替换天然dNTP，已经建立了用于更特异性DNA聚合的简单策略。
2 '-Deoxynucleoside 5 '-(alpha-P-seleno)-triphosphates (dNTP alpha Se) have been conveniently synthesized using a protection-free, one-pot strategy. One of two diastereomers of each dNTP alpha Se can be efficiently recognized by DNA polymerases, while the other is neither a substrate nor an inhibitor. Furthermore, this Se-atom modification can significantly inhibit non-specific DNA polymerization caused by mis-priming. Se-DNAs amplified with dNTP alpha Se via polymerase chain reaction have sequences identical to the corresponding native DNA. In conclusion, a simple strategy for more specific DNA polymerization has been established by replacing native dNTPs with dNTP alpha Se.