How far is single-cell sequencing from clinical application?
How far is single-cell sequencing from clinical application?
复制标题
单细胞测序距离临床应用还有多远？
DOI：
10.1002/ctm2.117
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发表时间：
2020
影响因子：
10.6
通讯作者：
Zeng Yiming
中科院分区：
文献类型：
--
作者：
Zhang Jiaqiang;Wang William;Huang Jianan;Wang Xiangdong;Zeng Yiming
单细胞测序(SC-seq)正迅速成为单细胞生物医学的重要方法之一，其核心是单细胞RNA测序(scRNA-seq)和单细胞DNA测序(scDNA-seq)。越来越多的证据表明，SC-SEQ可以显著加快识别新的细胞类型、细胞间异质性、基于功能的亚类、智能细胞和人类细胞标记物、疾病生物标志物、治疗靶点，并可以评估细胞内和细胞间的沟通。1-3从转录组到染色质可及性，从DNA突变到组蛋白修饰，从基因组序列到多维，从表现组到功能，从动态到空间变化，揭示了单细胞的分子特征。Sc-seq是一种有助于理解rna-dna-蛋白质、蛋白质-蛋白质、细胞-细胞和病原体-宿主细胞之间的相互作用和交流的方法。
Single-cell sequencing (SC-seq) is rapidly becoming one of the most important approaches to single-cell biomedicine and is centered around single-cell RNA sequencing (scRNA-seq) and single-cell DNA sequencing (scDNA-seq). Growing evidence suggests that SC-seq can significantly speed up the identification of new cell types, intercellular heterogeneity, function-based sub-categories, intelligent cell and human cell altars, disease biomarkers, therapeutic targets, and it can evaluate intra-and intercellular communication. 1-3 Molecular characteristics of single cells are uncovered through transcriptome to chromatin accessibility, DNA mutation to histone modification, genome sequences to multi-dimensions, phenomes to functions, and from dynamics to spatial alternations. SC-seq is an approach that contributes to the understanding of interactions and communications between RNA-DNA-proteins, proteins-proteins, cells-cells, and pathogens-host cells from which molecular mechanisms