Tamoxifen promotes apoptosis and inhibits invasion in estrogen-positive breast cancer MCF-7 cells
Tamoxifen promotes apoptosis and inhibits invasion in estrogen-positive breast cancer MCF-7 cells
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DOI：
10.3892/mmr.2017.6603
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发表时间：
2017-07-01
影响因子：
3.4
通讯作者：
Zhu, Ran
中科院分区：
文献类型：
--
作者：
Li, Wei;Shi, Xingpeng;Zhu, Ran
三苯氧胺（Tamoxifen，TAM）是最早的非甾体类抗雌激素药物，已广泛应用于乳腺癌的内分泌治疗。本研究旨在探讨TAM对雌激素阳性（ER+）乳腺癌细胞株MCF-7增殖、凋亡、迁移和侵袭的影响，并探讨其作用机制。TAM能抑制MCF-7细胞的增殖、迁移和侵袭，并诱导其凋亡。进一步研究发现，TAM处理MCF-7细胞后，线粒体膜电位和ATP含量显著降低。线粒体是活性氧的重要来源，也是活性氧的作用靶点。在本研究中，TAM促进MCF-7细胞中ROS的形成。总之，这些结果揭示了TAM诱导ER+乳腺癌细胞凋亡和抑制侵袭的潜在机制，从而支持TAM在乳腺癌治疗中的应用。
Tamoxifen (TAM) is the earliest non-steroidal antiestrogen drug, which has been widely used in endocrine therapy targeting breast cancer. The aim of the present study was to investigate the effect of TAM on the proliferation, apoptosis, migration and invasion of the estrogen-positive (ER+) breast cancer cell line MCF-7 in vitro, and elucidate its mechanisms. It was demonstrated that TAM suppressed proliferation, migration and invasion, and induced apoptosis in MCF-7 cells. Further investigation revealed that the mitochondrial membrane potential and the amount of ATP were significantly decreased following the treatment of MCF-7 cells with TAM. Mitochondria are an important source of reactive oxygen species (ROS) and they are also the target of ROS as well. In the present study, TAM promoted the formation of ROS in MCF-7 cells. In conclusion, these results reveal the underlying mechanism by which TAM induces ER+ breast cancer cell apoptosis and inhibits invasion, thereby supporting the use of TAM in breast cancer treatment.