Creation of cross-linked bilayer membranes that can incorporate membrane proteins from oligo-asp-based Peptide gemini surfactants.
Creation of cross-linked bilayer membranes that can incorporate membrane proteins from oligo-asp-based Peptide gemini surfactants.
复制标题
创建交联双层膜，该膜可以结合来自基于寡天冬氨酸的肽双子表面活性剂的膜蛋白。
作者：
Koeda;S. ; Umezaki;K. ; Sumino;A. ; Noji;T. ; Ikeda;A. ; Yamamoto;Y. ; Dewa;T. ; Taga;K. ; Nango;M. ; Tanaka;T. ; Mizuno;T.
我们设计了一种新型的双层形成的两亲体，基于环寡聚Asp基肽gemini （PG）表面活性剂cr- d2c12和cr-D3C12，该两亲体由- Cys(Asp)nCys - （n= 2或3）作为核心肽和两个含有十二烷基酰胺甲基的Cys残基组成。动态光散射和透射电镜测量显示，形成了球形双层膜，可以吸收来自嗜酸红假单胞菌的光收集天线复合物2 （LH2）。此外，这种蛋白脂质体样的缀合物可以组装在阳离子化的玻璃和云母上，形成含有LH2的平面双层膜。利用原子力显微镜，我们观察到LH2从由cr- d2c12或cr-D3C12形成的平面双层膜的平台上突出（约1.2-1.5 nm）。因此，我们设计的PG表面活性剂是一类新的双层形成的两亲分子，可以应用于各种膜蛋白的研究。
We designed novel bilayer-forming amphiphiles based on the cyclic oligo-Asp-based peptide gemini (PG) surfactants cr-D2C12and cr-D3C12, which consist of −Cys(Asp)nCys– (n= 2 or 3) as a core peptide and two Cys residues containing a dodecylamidomethyl group. Dynamic light scattering and transmission electron microscopy measurements revealed the formation of spherical bilayer membranes that could incorporate the light-harvesting antenna complex 2 (LH2) from Rhodopseudomonas acidophila. Furthermore, this proteoliposome-like conjugate could be assembled onto cationized glass and mica to form planar bilayer membranes incorporating LH2. Using atomic force microscopy, we observed LH2 protruding (ca. 1.2–1.5 nm) from flat terraces of the planar bilayer membranes formed from cr-D2C12or cr-D3C12. Thus, our designed PG surfactants are a new class of bilayer-forming amphiphiles that may be applied to the study of various membrane proteins.