Use of Bordetella pertussis BP3385 To Establish a Cutoff Value for an IS481-Targeted Real-Time PCR Assay

Use of Bordetella pertussis BP3385 To Establish a Cutoff Value for an IS481-Targeted Real-Time PCR Assay
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DOI:
10.1128/jcm.01551-08
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发表时间:
2008-11-01
影响因子:
9.4
通讯作者:
Drews, S. J.
Drews, S. J.
中科院分区:
医学2区
文献类型:
--
作者:
Guthrie, J. L.;Seah, C.;Drews, S. J.

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本研究利用百日咳博德特氏菌单拷贝PCR靶标BP 3385作为确认循环阈值(C-T)值>35的IS 481 PCR阳性反应的方法。C-T值>35个循环的IS 481 PCR可能代表存在以下情况的PCR条件:
This study utilized the Bordetella pertussis single-copy PCR target BP3385 as a means of confirming IS481 PCR-positive reactions with cycle threshold (C-T) values of >35. IS481 PCRs with C-T values of >35 cycles may represent PCR conditions where there is