The amino terminus of the adenovirus fiber protein encodes the nuclear localization signal.
The amino terminus of the adenovirus fiber protein encodes the nuclear localization signal.
复制标题
腺病毒纤维蛋白的氨基末端编码核定位信号。
DOI：
10.1016/0042-6822(91)90547-o
复制
发表时间：
1991
期刊：
影响因子：
3.7
通讯作者：
Engler,JA
中科院分区：
文献类型：
--
作者：
Hong,JS;Engler,JA
利用重组痘苗病毒载体，在人细胞中表达了2型腺病毒纤维蛋白；表达的蛋白质被正确地组装成三聚体，糖基化，并运输到细胞核。氨基酸2-5 （KRAR）的缺失导致细胞质中纤维的积累；将Adz纤维起始的TKRVRL序列融合到该突变体的n端，恢复了正确的靶向性。改变另一个潜在靶向序列（LKKTK到LEETK）中的氨基酸91和92的电荷对核靶向的影响很小。当融合到β-半乳糖苷酶的n端并在重组痘苗病毒中表达时，无论是MKRARP还是MTKRVRL（分别来自Ad2和Ad7纤维）都不足以有效地将杂交蛋白转运到细胞核；另一方面，MKRARPSEDTF（来自Ad2纤维）或MKRPRP（来自Ad2 El a蛋白c端已知的靶向序列）与β-半乳糖苷酶的融合定位于细胞核。这些结果表明，Ad2和Ad7纤维的n端序列是正确的核靶向所必需的。
Using a recombinant vaccinia virus vector, the fiber protein from adenovirus serotype 2 has been expressed in human cells; the protein expressed was correctly assembled into trimers, glycosylated, and transported to the nucleus. Deletion of amino acids 2–5 (KRAR) resulted in accumulation of fiber in the cytoplasm; fusion of the sequence TKRVRL, found at the beginning of Adz fiber, to the N-terminus of this mutant restored correct targeting. Changing the charge of amino acids 91 and 92 within another potential targeting sequence (LKKTK to LEETK) had little effect on nuclear targeting. When fused to the N-terminus of β-galactosidase and expressed in recombinant vaccinia virus, neither MKRARP nor MTKRVRL (from Ad2 and Ad7 fibers, respectively), were sufficient for efficient transport of the hybrid protein to the nucleus; on the other hand, fusions of either MKRARPSEDTF (from Ad2 fiber) or of MKRPRP (a known targeting sequence from the C-terminus of Ad2 El A proteins) to β-galactosidase were localized to the nucleus. These results suggest that sequences at the N-terminus of Ad2 and Ad7 fiber are required for correct nuclear targeting.