Allosteric and non-allosteric E. coli phosphofructokinases: effects on growth.
Allosteric and non-allosteric E. coli phosphofructokinases: effects on growth.
复制标题
变构和非变构大肠杆菌磷酸果糖激酶：对生长的影响。
DOI：
10.1016/0006-291x(78)91430-4
复制
发表时间：
1978
影响因子：
3.1
通讯作者：
D. Fraenkel
中科院分区：
文献类型：
--
作者：
J. Robinson;D. Fraenkel
在野生型大肠杆菌K-12 ca.已知90%的磷酸果糖激酶是变构酶Pfk-1，其余的是Pfk-2，一种非变构酶。现已构建了具有不同组合和Pfk-1和Pfk-2量的等基因菌株系列（例如，无Pfk-1，高水平Pfk-2;正常水平Pfk-1，高水平Pfk-2等）。在含有葡萄糖、葡萄糖-6-P和甘油的基本培养基中，在有氧和厌氧条件下，只要有足够总量的酶，那么它是什么变构类型对该生物体的生长速率或产量没有太大影响。
In wild type Escherichia coli K-12 ca. 90% of phosphofructokinase is known to be the allosteric enzyme Pfk-1, and the rest is Pfk-2, a non-allosteric enzyme. An isogenic strain series has now been constructed with varying combinations and amounts of Pfk-1 and Pfk-2 (eg, no Pfk-1, high level of Pfk-2; normal level Pfk-1, high level Pfk-2, etc.). In minimal medium with glucose, glucose-6-P, and glycerol, aerobically and anaerobically, provided there is adequate total amount of enzyme, what allosteric type it is does not make much difference to growth rate or yield of this organism.