Exploiting the Interaction of Metal Ions and Peptide Nucleic Acids-DNA Duplexes for the Detection of a Single Nucleotide Mismatch by Electrochemical Impedance Spectroscopy
Exploiting the Interaction of Metal Ions and Peptide Nucleic Acids-DNA Duplexes for the Detection of a Single Nucleotide Mismatch by Electrochemical Impedance Spectroscopy
复制标题
利用金属离子和肽核酸-DNA 双链体的相互作用通过电化学阻抗谱检测单核苷酸不匹配
DOI：
10.1021/ac902813y
复制
发表时间：
2010-02-01
影响因子：
7.4
通讯作者：
Kraatz, Heinz-Bernhard
中科院分区：
文献类型：
--
作者：
Li, Congjuan;Li, Xiaohong;Kraatz, Heinz-Bernhard
以[Fe（CN）（6）]（3-/4-）为氧化还原探针，用电化学阻抗谱研究了金属离子Mg ~（2+）、Zn ~（2+）、Ni ~（2+）和Co ~（2+）与金表面DNA-肽核酸（PNA）膜的相互作用.阻抗数据进行了分析的帮助下修改的Randles的等效电路。电荷转移电阻R-CT的变化顺序为Ni 2 + > Co 2 + > Zn 2 + > Mg 2+。我们解释这些结果的Ni 2+与DNA-PNA膜相比，其他金属离子，可能涉及与核碱基的相互作用，可能与嘌呤的N7或嘧啶的N3更强的相互作用。在这些观察的基础上，选择Ni 2+来探测15-mer PNA-DNA膜中C-T错配的检测。使用Ni 2+，可以检测单个C-T失配。与相同的15-mer DNA-DNA杂交体相比，PNA-DNA杂交体的所得Δ R-CT更大。
The interaction of the metal ions Mg2+, Zn2+, Ni2+, and Co2+ with DNA-peptide nucleic acid (PNA) films on a gold surface is studied by electrochemical impedance spectroscopy in the presence of [Fe(CN)(6)](3-/4-) as the redox probe. Impedance data were analyzed with the help of a modified Randles' equivalent circuit. Changes in the charge-transfer resistance, R-CT, decreases in the order of Ni2+ > Co2+ > Zn2+ > Mg2+. We interpret these results in terms of stronger interactions for Ni2+ with the DNA-PNA film compared to the other metal ions, potentially involving interactions with the nucleobases, presumably with the N7 of purines or the N3 of pyrimidines. On the basis of these observations, Ni2+ was chosen to probe the detection of a C-T mismatch in 15-mer PNA-DNA films. Using Ni2+, it is possible to detect a single C-T mismatch. The resulting Delta R-CT is larger for the PNA-DNA hybrid compared to that for the identical 15-mer DNA-DNA hybrid.