Electrophoresis of proteins in intercellular bridges
Electrophoresis of proteins in intercellular bridges
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DOI：
10.1038/286084a0
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发表时间：
1980-07
期刊：
影响因子：
64.8
通讯作者：
R. I. Woodruff;W. Telfer
中科院分区：
文献类型：
--
作者：
R. I. Woodruff;W. Telfer
Jaffe和他的同事已经提出，在有丝分裂时，具有不同但互补形态发生功能的子细胞的细胞质极性具有跨细胞离子电流作为其基本生理步骤之一。最明显的证据来自岩藻卵子，这种电流已经被证明平行于预期的发芽轴2，3。电流是由细胞一侧的阳离子泵和相反一侧的渗透通道的稳定积累引起的；它们足够强，足以表明伴随的电位梯度可能对细胞质成分的分布产生电泳效应，包括那些作为形态发生决定因素的成分。尽管与形态发生轴相关的离子电流已被明确确立，但它们对细胞内定位和形态发生活性的影响仍然是推测的5。我们在这里报道了内源产生的电势梯度在卵母细胞-哺育细胞合体中实际上影响了细胞质连续体中可溶性蛋白质的分布。
Cytoplasmic polarity giving rise at mitosis to daughter cells with distinct but complementary morphogenetic functions has been proposed by Jaffe and co-workers to have transcellular ion currents as one of its essential physiological steps1. The clearest evidence is from fucoid eggs in which such currents have been shown to parallel the prospective axis of germination2,3. The currents are caused by stabilized accumulations of cation pumps on one side of the cell, and of permeability channels on the opposite side4; they are strong enough to suggest that the accompanying gradient in electrical potential could have an electrophoretic effect on the distribution of cytoplasmic constituents, including those that serve as morphogenetic determinants. Although ion currents correlated with axes of morphogenesis have thus been clearly established, their effects on intracellular localizations and morphogenetic activity have remained speculative5. We report here evidence that an endogenously generated gradient in electrical potential in the oocyte–nurse cell syncytium ofHyalophora cecropiacan, in fact, influence the distribution of soluble proteins in a cytoplasmic continuum.