The impact of oxidation on spore and pollen chemistry
The impact of oxidation on spore and pollen chemistry
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DOI：
10.1144/jmpaleo2014-022
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发表时间：
2015-07-01
影响因子：
2
通讯作者：
Gosling, William D.
中科院分区：
文献类型：
--
作者：
Jardine, Phillip E.;Fraser, Wesley T.;Gosling, William D.
孢子形态（花粉和孢子）具有由孢粉素组成的外壁。孢粉素化学包含环境紫外线-B通量和分类信息的签名，但目前还不知道这是如何敏感的标准孢粉处理技术。特别是已知氧化会导致孢子形态的物理降解，预计这对孢粉素化学具有一致的影响。在这里，我们测试实验氧化石松（clubmoss）孢子使用两种常见的氧化技术：乙酰和硝酸。我们还进行了八个被子植物（显花植物）类群的乙酰解，以测试我们的结果的一般性。使用傅里叶变换红外（FTIR）光谱，我们发现，乙酰化去除不稳定的，非易失性成分的sporomorphs，但有一个有限的影响，在正常的处理时间后的孢粉素的化学。硝酸更具侵略性，确实可以分解孢粉素并重组其化学结构，但当仅限于短期处理时（即
Sporomorphs (pollen and spores) have an outer wall composed of sporopollenin. Sporopollenin chemistry contains both a signature of ambient ultraviolet-B flux and taxonomic information, but it is currently unknown how sensitive this is to standard palynological processing techniques. Oxidation in particular is known to cause physical degradation to sporomorphs, and it is expected that this should have a concordant impact on sporopollenin chemistry. Here, we test this by experimentally oxidizing Lycopodium (clubmoss) spores using two common oxidation techniques: acetolysis and nitric acid. We also carry out acetolysis on eight angiosperm (flowering plant) taxa to test the generality of our results. Using Fourier Transform infrared (FTIR) spectroscopy, we find that acetolysis removes labile, non-fossilizable components of sporomorphs, but has a limited impact upon the chemistry of sporopollenin under normal processing durations. Nitric acid is more aggressive and does break down sporopollenin and reorganize its chemical structure, but when limited to short treatments (i.e.