Enhancing Nanos expression via the bacterial TomO protein is a conserved strategy used by the symbiont Wolbachia to fuel germ stem cell maintenance in infected Drosophila females.
Enhancing Nanos expression via the bacterial TomO protein is a conserved strategy used by the symbiont Wolbachia to fuel germ stem cell maintenance in infected Drosophila females.
复制标题
通过细菌 TomO 蛋白增强 Nanos 表达是共生体沃尔巴克氏体用来促进受感染果蝇雌性生殖干细胞维持的保守策略。
DOI：
10.1002/arch.21471
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Yamamoto D
中科院分区：
文献类型：
--
作者：
Ote M;Yamamoto D
在与醋蝇黑腹果蝇共生的沃尔巴克氏体（Wolbachia）的wMel株中，已经鉴定出卵子发生毒性操纵子（TomO）蛋白，作为影响宿主生殖的蛋白。TomO保护生殖干细胞（GSC）免于变性，否则变性发生在对于基因Sex-致死（Sxl）突变的宿主雌性的卵巢中。我们从致倦库蚊的Wolbachia菌株wPip中分离到TomO同源物。其中一个同源物TomOwPip 1在缺乏其疏水延伸时在flySxlmutants中发挥GSC拯救活性。TomOwPip 1变体的GSC拯救作用归因于其与Nanos（nos）mRNA结合并增强Nos蛋白表达的能力。对TomO同源物和TomO缺失变体的结构-活性关系的分析揭示了蛋白质中的不同模块，每个模块都致力于不同的功能，即，亚细胞定位、nosmRNA结合或Nos表达增强。我们建议，模块化改组是TomO蛋白成员的结构和功能多样化的基础。
The toxic manipulator of oogenesis (TomO) protein has been identified in thewMel strain ofWolbachiathat symbioses with the vinegar flyDrosophila melanogaster, as a protein that affects host reproduction. TomO protects germ stem cells (GSCs) from degeneration, which otherwise occurs in ovaries of host females that are mutant for the geneSex‐lethal(Sxl). We isolated the TomO homologs fromwPip, aWolbachiastrain from the mosquitoCulex quinquefasciatus. One of the homologs, TomOwPip1, exerted the GSC rescue activity in flySxlmutants when lacking its hydrophobic stretches. The GSC‐rescuing action of the TomOwPip1 variant was ascribable to its abilities to associate with Nanos (nos) mRNA and to enhance Nos protein expression. The analysis of structure–activity relationships with TomO homologs and TomO deletion variants revealed distinct modules in the protein that are each dedicated to different functions, i.e., subcellular localization,nosmRNA binding or Nos expression enhancement. We propose that modular reshuffling is the basis for structural and functional diversification of TomO protein members.