Identification of quantitative trait loci function through analysis of multiple cuticular hydrocarbons differing between Drosophila simulans and Drosophila sechellia females
Identification of quantitative trait loci function through analysis of multiple cuticular hydrocarbons differing between Drosophila simulans and Drosophila sechellia females
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DOI：
10.1038/hdy.2009.79
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发表时间：
2009-11-01
期刊：
影响因子：
3.8
通讯作者：
Ritchie, M. G.
中科院分区：
文献类型：
--
作者：
Gleason, J. M.;James, R. A.;Ritchie, M. G.
性隔离的遗传学，物种之间阻止交配的行为差异，知之甚少。物种间信息素的差异可以影响许多动物的性隔离，在某些情况下，一个单一的位点可以引起大的功能变化的信息素交配信号。果蝇表皮碳氢化合物（CHCs）可作为信息素影响其配偶识别。在以前的研究中，两个主要的CHCs在女性之间的交配歧视影响果蝇simulans和D。sechellia，数量性状位点（QTL）上的X和第三染色体上，和一些候选基因可能涉及。在这里，我们专门测试候选基因的CHC生物合成，并确定了四个额外的CHC的遗传结构，不同的丰度之间的D。simulans和D. sechellia女性。相同的QTL，和新的，被发现为额外的CHCs。通过检查所有这些CHC并探索其协方差，我们能够将推定的功能归因于主效QTL。尽管去饱和酶在CHC生物合成中的作用受到了相当大的关注，但这里的证据表明，延伸酶可能同样重要。性别决定基因似乎没有在这种物种差异的作用，虽然D。sechellia在CHCs中表现为性二型，而D. simulans不是。上位相互作用，仅检测到限于D的CHC。sechellia，意味着基因座之间复杂的相互作用也可能在这些影响交配隔离的化合物中起作用。Heredity（2009）103，416-424; doi：10.1038/hdy.2009.79;在线发表2009年8月5日
The genetics of sexual isolation, behavioral differences between species that prevent mating, is understood poorly. Pheromonal differences between species can influence sexual isolation in many animals and in some cases a single locus can cause large functional changes in pheromonal mating signals. Drosophila cuticular hydrocarbons (CHCs) can function as pheromones and consequently affect mate recognition. In a previous study of the two major CHCs in females that affect mating discrimination between Drosophila simulans and D. sechellia, quantitative trait loci (QTL) were identified on the X and third chromosome, and a few candidate genes were potentially implicated. Here we specifically test candidate genes for CHC biosynthesis and determine the genetic architecture of four additional CHCs that differ in abundance between D. simulans and D. sechellia females. The same QTL, and new ones, were found for additional CHCs. By examining all these CHCs and exploring their covariance, we were able to ascribe putative function to the major QTL. Although desaturases have received considerable attention for their role in CHC biosynthesis, evidence here implies that elongases may be just as important. Sex determination genes do not seem to have a role in this species difference although D. sechellia is sexually dimorphic in CHCs, whereas D. simulans is not. Epistatic interactions, only detected for CHCs limited to D. sechellia, imply that complex interactions among loci may also be having a role in these compounds that affect mating isolation. Heredity ( 2009) 103, 416-424; doi: 10.1038/hdy.2009.79; published online 5 August 2009