Analysis of quantitative trait loci (QTLs) and quantitative trait alleles (QTAs) for potato tuber yield and starch content
Analysis of quantitative trait loci (QTLs) and quantitative trait alleles (QTAs) for potato tuber yield and starch content
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DOI：
10.1007/s001220050963
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发表时间：
1998-10-01
影响因子：
5.4
通讯作者：
Gebhardt, C
中科院分区：
文献类型：
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作者：
Schäfer-Pregl, R;Ritter, E;Gebhardt, C
利用RFLP标记，在两个马铃薯非自交系杂交F-1群体中定位了控制块茎淀粉含量和块茎产量的QTL。在单标记检验和区间分析的基础上，首次成功地将非自交系植物的区间QTL分析模型应用于试验数据。两种方法的QTL分析结果相似，但不完全相同。分别在5个环境和2个环境中对分离群体K31和Li-I的块茎淀粉含量和块茎产量进行了QTL分析。群体K31是全基因分型群体，而群体LPI是根据块茎淀粉含量高低进行选择性基因分型。在12个马铃薯连锁群上检测到18个可能控制块茎淀粉含量的QTL，在8个连锁群上检测到8个可能控制块茎产量的QTL。在26个可能的QTL中，有26个在至少两个环境和/或作图群体中被重复检测到。控制块茎淀粉含量的主效QTL在不同环境中高度稳定，但只在所分析的两个作图群体中的一个群体中检测到。多数控制块茎产量的QTL与控制块茎淀粉含量的QTL连锁，表明对这两个性状的影响受相同的遗传因素控制。并就马铃薯分子标记辅助选择的研究结果进行了讨论。
Using RFLP markers, QTLs for tuber starch-content and tuber yield were mapped in two F-1 populations derived from crossing non-inbred di-haploid potato breeding lines. QTLs were identified and mapped, based on both single-marker tests and interval analyses, A model specifically developed for interval QTL analysis in non-inbred plant species was successfully applied for the first time to experimental data. Results of both methods of QTL analysis were similar but not identical. QTLs for tuber starch-content and tuber yield were analysed in segregating populations K31 and LI-I in five and two environments, respectively. Population K31 was fully genotyped whereas population LPI was selectively genotyped according to high and low tuber-starch content. Eighteen putative QTLs for tuber starch-content were identified on all 12 potato linkage groups and eight putative QTLs for tuber yield were identified on eight linkage groups. Twenty of twenty six putative QTLs were reproducibly detected in at least two environments and/or mapping populations. Few major QTLs for tuber starch-content were highly stable across environments but were detected in only one of the two mapping populations analysed. Most QTLs for tuber yield were linked with QTLs for tuber starch-content suggesting that the effects on both traits are controlled by the same genetic factors. The results are discussed with respect to marker-assisted selection in potato.