Pretreatment of spent mushroom substrate for enhancing the conversion of fermentable sugar
Pretreatment of spent mushroom substrate for enhancing the conversion of fermentable sugar
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废蘑菇底物的预处理以提高可发酵糖的转化
DOI：
10.1016/j.biortech.2013.08.122
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发表时间：
2013-11-01
影响因子：
11.4
通讯作者：
Zou, Shuangquan
中科院分区：
文献类型：
--
作者：
Wu, Songqing;Lan, Yanjiao;Zou, Shuangquan
为了开发一种具有成本效益的生物农药，研究了废蘑菇底物（SMS）提取物作为培养苏云金芽孢杆菌（Bt）的潜在碳源。比较了几种预处理方法，确定了纤维素降解生产还原糖的最佳方法，包括稀硫酸（0.5-2.0% v/v, 50-121℃，1 h）、氢氧化钠（0.5-2% w/v, 50-121℃，1 h）、氢氧化钙（0.2-4% w/v, 50-121℃，1 h）和热水（50-121℃，1 h）。预处理后进行标准酶解和发酵。结果表明，采用2%稀硫酸在121℃条件下预处理1 h，纤维素降解效果最好，还原糖得率最高（284.24 g/kg SMS）。同样的预处理条件下产孢量也最高。SMS的使用不仅是bt生物农药商业化的一种替代方式，而且是解决蘑菇产业废底物积累带来的环境污染的潜在解决方案。(C) 2013 Elsevier Ltd.版权所有。
To develop a cost-effective biopesticide, spent mushroom substrate (SMS) extract was studied as a potential carbon source for cultivating Bacillus thuringiensis (Bt). Several pretreatments were compared to determine the optimal method for degrading cellulose to produce reducing sugars, including dilute sulfuric acid (0.5-2.0% v/v, 50-121 degrees C, 1 h), sodium hydroxide (0.5-2% w/v, 50-121 degrees C, 1 h), calcium hydroxide (0.2-4% w/v, 50-121 degrees C, 1 h), and hot water (50-121 degrees C, 1 h). Pretreatment was followed by standard enzymatic hydrolysis and fermentation. Results showed that the highest cellulose degradation was obtained using 2% dilute sulfuric acid pretreatment at 121 degrees C for 1 h, resulting in a high yield of reducing sugar (284.24 g/kg SMS). Sporulation was also highest using the same pretreatment. Use of SMS is not only an alternative way to commercialize Bt-based biopesticide, but also a potential solution for the environmental pollution associated with accumulation of the spent substrate of the mushroom industry. (C) 2013 Elsevier Ltd. All rights reserved.