Responses of rose RhACS1 and RhACS2 promoters to abiotic stresses in transgenic Arabidopsis thaliana
Responses of rose RhACS1 and RhACS2 promoters to abiotic stresses in transgenic Arabidopsis thaliana
复制标题
转基因拟南芥中玫瑰 RhACS1 和 RhACS2 启动子对非生物胁迫的响应
DOI：
10.1007/s00299-015-1742-8
复制
发表时间：
2015-05-01
影响因子：
6.2
通讯作者：
Ma, Nan
中科院分区：
文献类型：
--
作者：
Khan, Muhammad Ali;Meng, Yonglu;Ma, Nan
RhACS 1和RhACS 2是两个参与乙烯生物合成的玫瑰基因，其启动子活性对各种非生物胁迫具有高度的器官特异性。我们以前的研究表明，两个玫瑰（玫瑰杂交）1-氨基环丙烷-1-羧酸合酶基因，RhACS 1和RhACS 2，在脱水诱导的乙烯产生和抑制细胞扩张的玫瑰花瓣中发挥作用。在这里，两个RhACS 1和RhACS 2启动子进行了分析，使用组织化学染色和葡萄糖醛酸酶合酶（GUS）基因报告活性测定后，将其引入到转基因拟南芥植物。结果表明，这两个基因的启动子活性在从幼苗到成熟开花植物的下胚轴、子叶、叶、根和侧根等器官中均表现出较强的活性。干旱诱导转基因拟南芥莲座叶和根中RhACS 1启动子的活性。thaliana lines，但在再水化处理后减少。与此相反，RhACS 2启动子活性下降，干旱莲座叶，而其响应模式是类似的RhACS 1在根中。甘露醇处理诱导的活性的RhACS 1和RhACS 2启动子，表明这两个基因也受到渗透胁迫。此外，RhACS 2似乎是脱落酸（阿坝）诱导的，而RhACS 1对阿坝不太敏感。最后，四个截短序列的RhACS 1启动子和GUS活性测定表明，删除之间的bp 862和-535之间的327 bp的区域导致启动子活性的显着降低。两者合计，我们的研究结果表明，RhACS 1和RhACS 2启动子响应非生物胁迫的发育调节和空间特异性的方式。
Promoter activities of RhACS1 and RhACS2 , two rose genes involved in ethylene biosynthesis, are highly sensitive to various abiotic stresses in an organ-specific manner. Our previous studies indicated that two rose (Rosa hybrida) 1-aminocyclopropane-1-carboxylic acid synthase genes, RhACS1 and RhACS2, play a role in dehydration-induced ethylene production and inhibition of cell expansion in rose petals. Here, both RhACS1 and RhACS2 promoters were analyzed using histochemical staining and glucuronidase synthase (GUS) gene reporter activity assays following their introduction into transgenic Arabidopsis thaliana plants. It was found that the promoter activities of both genes were strong throughout the course of development from young seedlings to mature flowering plants in various organs, including hypocotyls, cotyledons, leaves, roots and lateral roots. RhACS1 promoter activity was induced by drought in both rosette leaves and roots of transgenic A. thaliana lines, but was reduced following a re-hydration treatment. In contrast, RhACS2 promoter activity was decreased by drought in rosette leaves, while its response pattern was similar to that of RhACS1 in roots. A mannitol treatment induced the activity of both the RhACS1 and RhACS2 promoters, indicating that both genes are also regulated by osmotic stress. In addition, RhACS2 appeared to be abscisic acid (ABA)-inducible, while RhACS1 was less sensitive to ABA. Finally, four truncated sequences of the RhACS1 promoter were generated and GUS activity assays demonstrated that deleting a 327 bp region between bp 862 and -535 resulted in a substantial decrease of the promoter activity. Taken together, our results suggest that the RhACS1 and RhACS2 promoters respond to abiotic stresses in a developmentally regulated and spatially specific manner.