Ratiometric Molecular Sensor for Monitoring Oxygen Levels in Living Cells
Ratiometric Molecular Sensor for Monitoring Oxygen Levels in Living Cells
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DOI：
10.1002/anie.201107557
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发表时间：
2012-01-01
影响因子：
16.6
通讯作者：
Tobita, Seiji
中科院分区：
文献类型：
--
作者：
Yoshihara, Toshitada;Yamaguchi, Yuji;Tobita, Seiji
氧（双氧）是好氧生物的主要代谢物之一在细胞呼吸过程中，氧作为电子传递链和氧化磷酸化的终端受体起着重要作用。缺氧与多种疾病有关，并发生在肿瘤微环境中因此，定量生物细胞和组织中的氧水平对于了解细胞功能（功能障碍）和评估癌症治疗中药物输送过程中的肿瘤病理生理至关重要。我们最近证明了磷光铱-(III)配合物可以用作光学传感器，用于可视化生物细胞和组织中的氧水平我们使用一种红发铱配合物[(btp) 2Ir (acac)]（btp; bis（2-（2′-苯并噻吩基）-吡啶- n， C3′）铱（乙酰丙酮））进行光学成像，因为该化合物在脱氧己烷中具有中等长的发射寿命（6.3 μs）和高量子产率（0.31）。BTP的红光被溶液中的溶解氧显著猝灭。在活细胞中也观察到类似的氧猝灭现象。在低氧压力下用BTP培养人宫颈癌细胞，其发射图像较亮，反映了细胞的含氧量水平。除了强度测量外，通常还需要发射寿命测量来量化细胞和组织中的氧水平作为一种替代方法，比例氧传感器不需要专门的仪器来测量发射寿命，适用于一般测量，并将有利于细胞生物学家和医学科学家。比例氧传感器
Oxygen (dioxygen) is one of the key metabolites in aerobic organisms.[1] In cellular respiration, oxygen plays a major role as the terminal acceptor of the electron transport chain and oxidative phosphorylation. Oxygen deprivation (hypoxia) is connected with various diseases and occurs in tumor microenvironments.[2] It is thus essential to quantify oxygen levels in biological cells and tissues to understand cellular function (dysfunction) and to assess tumor pathophysiology during drug delivery in cancer therapies. We recently demonstrated that phosphorescent iridium-(III) complexes can be used as optical sensors for visualizing the oxygen levels in biological cells and tissues.[3] We used a red-emitting iridium complex [(btp) 2Ir (acac)](BTP; bis (2-(2’-benzothienyl)-pyridinato-N, C3’) iridium (acetylacetonate)) for optical imaging, because this compound has a moderately long emission lifetime (6.3 μs) and a high quantum yield (0.31) in deaerated hexane. The red phosphorescence of BTP is significantly quenched by dissolved oxygen in solution. Similar quenching by oxygen has also been observed for living cells. Emission images of HeLa (human cervical cancer) cells were brighter, when cells were cultured with BTP at low oxygen pressures, and thus the images reflected the cellular oxygen levels.In addition to intensity measurements, emission lifetime measurements are generally required to quantify oxygen levels in cells and tissues.[4] As an alternative method, ratiometric oxygen sensors, which do not require specialized instrumentation for measuring emission lifetimes, are suitable for general measurements and will be beneficial for cell biologists and medical scientists. Ratiometric oxygen sensors