Procedures for obtaining high percentages of viable in vitro capacitated hamster sperm
Procedures for obtaining high percentages of viable in vitro capacitated hamster sperm
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获得高百分比活体外获能仓鼠精子的程序
DOI：
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发表时间：
1979
期刊：
影响因子：
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通讯作者：
S. Meizel
中科院分区：
文献类型：
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作者：
C. W. Lui;R. Mrsny;S. Meizel
将洗净的金黄地鼠尾部精子通过0.25-0.3 mm的玻璃珠柱，制备了近100%可存活的金黄地鼠精子悬浮液。这些精子在体外获能条件下以0.1或1毫升(2-2.5×106/毫升)的体积孵育，4小时45分钟后获得85-92%的存活精子。其中70%以上的精子在孵育4小时45分钟后被判断为获得了能力，这是因为它们经历了顶体反应，并且存在大量表现出获得能力的仓鼠精子的激活运动特征的精子。
因此，这将是第一次出现这样的程序，它将产生大量可供生化分析使用的有能力存活的精子，这也将使其他关于仓鼠精子获能的研究能够最大限度地减少死精子释放出的分子的干扰。
Suspensions of nearly 100% viable golden hamster sperm were prepared by passing washed cauda epididymal sperm through a column of 0.25–0.3 mm glass beads. Incubations of these viable sperm under in vitro capacitation conditions in volumes of 0.1 or 1 ml (2–2.5 × 106/ml) resulted in 85–92% viable sperm after four hours and 45 minutes of incubation. More than 70% of these sperm were judged to have been capacitated after four hours and 45 minutes of incubation on the basis of their having undergone acrosome reactions and the presence of high numbers of sperm exhibiting the activated motility characteristic of capacitated hamster sperm.
Thus, for the first time, procedures are available that will yield large numbers of viable capacitated sperm for biochemical analysis and that will also allow other studies of hamster sperm capacitation with minimum interference from molecules released from dead sperm.