Preventive effects of low-intensity exercise on cancer cachexia-induced muscle atrophy
Preventive effects of low-intensity exercise on cancer cachexia-induced muscle atrophy
复制标题
低强度运动对癌症恶病质引起的肌肉萎缩的预防作用
作者：
Tanaka M;Sugimoto K;Fujimoto T;Xie K;Takahashi T;Akasaka H;Kurinami H;Yasunobe Y;Matsumoto T;Fujino H;Rakugi H
我们假设低强度耐力运动可能通过增加蛋白质合成和减少蛋白质降解来更有效地预防癌症恶病质诱导的肌肉萎缩。本研究的目的是评估低强度耐力运动对癌症恶病质性肌肉萎缩的影响，并阐明其机制。24只雄性Wistar大鼠随机分为4组：对照组（Cont）、对照组+运动组（Ex）、AH130诱导的癌症恶病质组（AH130）和AH130 +运动组（Ex）。通过腹腔注射AH130吉田腹水肝癌细胞诱导癌症恶病质；我们分析了比目鱼肌肌肉质量的变化以及骨骼肌蛋白降解和合成途径的主要调控因子或指标的基因和蛋白表达水平。低强度运动通过抑制泛素-蛋白酶体通路、增加缺氧诱导因子- 1α和磷酸化AMPK抑制肌肉质量损失，抑制哺乳动物比目鱼肌雷帕霉素通路靶细胞失活，有助于预防癌症恶病质诱导的肌肉萎缩。这些结果表明，低强度运动有可能成为预防癌症恶病质引起的肌肉萎缩的有效治疗干预措施。-Tanaka， M., Sugimoto， K., Fujimoto， T., Xie， K., Takahashi， T., Akasaka， H., Kurinami， H., yasube， Y., Matsumoto， T., Fujino， H., Rakugi， H.低强度运动对癌症恶病质诱导的肌肉萎缩的预防作用。中国生物医学工程学报，2016,32(2):444 - 444。www.fasebj.org
We hypothesized that low‐intensity endurance exercise might be more effective in preventing cancer cachexia–induced muscle atrophy through both an increase in protein synthesis and a decrease in protein degradation. The purpose of present study was to evaluate the effects and to clarify the mechanism of low‐intensity endurance exercise on cancer cachexia–induced muscle atrophy. Twenty‐four male Wistar rats were randomly divided into 4 groups: control (Cont), Cont plus exercise (Ex), AH130‐induced cancer cachexia (AH130), and AH130 plus Ex. Cancer cachexia was induced by intraperitoneal injections with AH130 Yoshida ascites hepatoma cells; we analyzed the changes in muscle mass and the gene and protein expression levels of major regulators or indicators of skeletal muscle protein degradation and synthesis pathway in the soleus muscles. Low‐intensity exercise inhibited the muscle mass loss through a suppression of the ubiquitin‐proteasome pathway, increased hypoxia‐inducible factor‐1α and phosphorylated AMPK, and inhibited the deactivation of mammalian target of rapamycin pathway in the soleus muscle, which contributed to the prevention of cancer cachexia–induced muscle atrophy. These results suggest that low‐intensity exercise has the potential to become an effective therapeutic intervention for the prevention of cancer cachexia–induced muscle atrophy.—Tanaka, M., Sugimoto, K., Fujimoto, T., Xie, K., Takahashi, T., Akasaka, H., Kurinami, H., Yasunobe, Y., Matsumoto, T., Fujino, H., Rakugi, H. Preventive effects of low‐intensity exercise on cancer cachexia–induced muscle atrophy. FASEB J. 33, 7852–7862 (2019). www.fasebj.org