Analysis of blood spots for polyfluoroalkyl chemicals
Analysis of blood spots for polyfluoroalkyl chemicals
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DOI：
10.1016/j.aca.2009.10.007
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发表时间：
2009-12-10
影响因子：
6.2
通讯作者：
Calafat, Antonia M.
中科院分区：
文献类型：
--
作者：
Kato, Kayoko;Wanigatunga, Amal A.;Calafat, Antonia M.
多氟烷基化学品（PFCs）已在世界各地的人类、环境和生态系统中被检测到。某些全氟化学品可能对发育和生殖产生毒性，这对儿童的健康尤其令人关切。在美国，一个婴儿的血液样本，称为“干血斑”（DBS），是从一个孩子出生后48小时内的脚跟坚持。DBS可用于评估产前暴露于PFCs。建立了一种基于在线固相萃取-高效液相色谱-同位素稀释串联质谱法测定DBS中全氟辛烷磺酸（PFOS）、全氟己烷磺酸、全氟辛酸（PFOA）和全氟壬酸的方法。使用一个完整DBS（类似于75 μ L血液）的分析检测限< 0.5 ng mL（-1）。为了验证该方法，我们分析了2007年5月在美国收集的98个DBS。在所有DBS中都检测到全氟辛烷磺酸和全氟辛酸，浓度在低纳克/毫升（-1）范围内。这些数据表明，DBS可能是一个合适的矩阵评估围产期接触全氟化学品，但需要更多的信息有关的采样和标本储存，以证明这些措施的效用评估暴露。由爱思唯尔公司出版
Polyfluoroalkyl chemicals (PFCs) have been detected in humans, in the environment, and in ecosystems around the world. The potential for developmental and reproductive toxicities of some PFCs is of concern especially to children's health. In the United States, a sample of a baby's blood, called a "dried blood spot" (DBS), is obtained from a heel stick within 48 h of a child's birth. DBS could be useful for assessing prenatal exposure to PFCs. We developed a method based on online solid phase extraction coupled with high performance liquid chromatography-isotope dilution tandem mass spectrometry for measuring four PFCs in DBS, perfluorooctane sulfonate (PFOS), perfluorohexane sulfonate, perfluorooctanoate (PFOA), and perfluorononanoate. The analytical limits of detection using one whole DBS (similar to 75 mu L of blood) were < 0.5 ng mL(-1). To validate the method, we analyzed 98 DBS collected in May 2007 in the United States. PFOS and PFOA were detected in all DBS at concentrations in the low ng mL(-1) range. These data suggest that DBS may be a suitable matrix for assessing perinatal exposure to PFCs, but additional information related to sampling and specimen storage is needed to demonstrate the utility of these measures for assessing exposure. Published by Elsevier B.V.