Inhibitory effect of Sargassum polysaccharide on oxidative stress induced by infectious bursa disease virus in chicken bursal lymphocytes.
Inhibitory effect of Sargassum polysaccharide on oxidative stress induced by infectious bursa disease virus in chicken bursal lymphocytes.
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DOI：
10.1016/j.ijbiomac.2011.06.019
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发表时间：
2011-11
影响因子：
8.2
通讯作者：
Ling Zhang;Ting‐Jun Hu;Hong-li Liu;Xue-hong Shuai
中科院分区：
文献类型：
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作者：
Ling Zhang;Ting‐Jun Hu;Hong-li Liu;Xue-hong Shuai
研究马尾藻多糖对传染性法氏囊病病毒（IBDV）诱导的鸡法氏囊淋巴细胞氧化应激的抑制作用。测定了IBDV和马尾藻多糖处理鸡法氏囊淋巴细胞中IL-1β、IL-8、IL-10、TNF-α、MCP-1、还原性谷胱甘肽和活性氧的水平，并测定了超氧化物歧化酶和谷胱甘肽过氧化物酶的活性。结果表明，鸡法氏囊淋巴细胞与IBDV在100 TCID50下孵育8h后出现氧化应激。马尾藻多糖通过增加还原性谷胱甘肽的数量，促进超氧化物歧化酶和谷胱甘肽过氧化物酶的活性，降低活性氧水平来抑制氧化应激。多糖还能提高感染IBDV细胞的IL-1β、IL-8、IL-10和TNF-α水平。上述结果提示马尾藻多糖通过提高鸡法氏囊淋巴细胞的抗氧化能力和细胞因子水平来抗感染。
The aim of this study was to investigate the inhibitory effect of Sargassum polysaccharide on oxidative stress induced by infectious bursa disease virus (IBDV) in chicken bursal lymphocytes. The levels of IL-1β, IL-8, IL-10, TNF-α, MCP-1, reduced glutathione and reactive oxygen species in chicken bursal lymphocytes treated with IBDV or both IBDV and Sargassum polysaccharide were measured, and the activities of superoxide dimutase and glutathione peroxidase were evaluated. Our results showed that oxidative stress appeared when chicken bursal lymphocytes were incubated with IBDV for 8h at 100 TCID50. Sargassum polysaccharide inhibited oxidative stress by increasing the amount of reduced glutathione, promoting the activities of superoxide dimutase and glutathione peroxidase and reducing the level of reactive oxygen species. The polysaccharide also raised IL-1β, IL-8, IL-10 and TNF-α levels in cells infected with IBDV. These findings suggest that Sargassum polysaccharide acts against infection by elevating antioxidant capacity and cytokine levels in chicken bursal lymphocytes.