Cellular binding, motion, and internalization of synthetic gene delivery polymers
Cellular binding, motion, and internalization of synthetic gene delivery polymers
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DOI：
10.1016/j.bbamcr.2007.07.009
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发表时间：
2007-10-01
影响因子：
5.1
通讯作者：
Payne, Christine K.
中科院分区：
文献类型：
--
作者：
Hess, Gaelen T.;Humphries, William H.;Payne, Christine K.
使用荧光显微镜，我们跟踪了多聚精氨酸和聚乙烯亚胺的细胞结合、表面运动和内化，这两种阳离子配体用于基因和蛋白质的传递。每个配体都与一个量子点络合，以提供一个光稳定的探针。外源DNA的转染被用来将观察到的运动与基因传递联系起来。细胞表面运动不依赖于硫酸基蛋白多糖，但依赖于胆固醇。细胞内化需要硫酸化的蛋白多糖和胆固醇。这些观察表明，硫酸化的蛋白多糖是阳离子配体的细胞受体，而不仅仅是被动结合部位。了解聚精氨酸和聚乙烯亚胺与质膜的相互作用可能有助于设计更有效的基因递送系统。(C)2007 Elsevier B.V.保留所有权利。
Using fluorescence microscopy we have tracked the cellular binding, surface motion, and internalization of polyarginine and polyethylenimine, cationic ligands used for gene and protein delivery. Each ligand was complexed with a quantum dot to provide a photostable probe. Transfection with exogenous DNA was used to relate the observed motion to gene delivery. Cell surface motion was independent of sulfated proteoglycans, but dependent on cholesterol. Cellular internalization required sulfated proteoglycans and cholesterol. These observations suggest that sulfated proteoglycans act as cellular receptors for the cationic ligands, rather than only passive binding sites. Understanding the interaction of polyarginine and polyethylenimine with the plasma membrane may assist in designing more efficient gene delivery systems. (C) 2007 Elsevier B.V. All rights reserved.