Mapping a new frontier; development of integrated cytogenetic maps in plants
Mapping a new frontier; development of integrated cytogenetic maps in plants
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DOI：
10.1007/s101420000013
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发表时间：
2000-09-01
影响因子：
2.9
通讯作者：
Cande, W. Zacheus
中科院分区：
文献类型：
--
作者：
Harper, Lisa C.;Cande, W. Zacheus
顾名思义，细胞遗传学图谱将遗传图谱的数据与染色体的实际细胞学特征（如着丝粒、球和最近的荧光原位杂交（FISH）信号）结合起来。遗传学和细胞学图谱的整合主要通过两种方式完成。第一个一般策略是创建染色体断裂点，然后使用显微镜确定其细胞学位置，并使用遗传技术确定其在遗传图谱上的位置。第二种策略是通过FISH将遗传映射序列直接杂交到染色体上。本综述的目的是提供一个概述的状态，这一领域的植物。我们回顾了细胞遗传学图谱的历史和用途，并根据我们所学到的知识讨论了未来的发展方向。
Cytogenetic maps, as the name implies, incorporate data from genetic maps with actual cytological features of chromosomes such as centromeres, knobs and, recently, fluorescence in situ hybridization (FISH) signals. Integration of genetic and cytological maps has been accomplished primarily in two ways. The first general strategy is to create a chromosome breakpoint, then determine its cytological position using microscopy, and its position on the genetic map using genetic techniques. A second strategy is by the direct hybridization of genetically mapped sequences onto chromosomes by FISH. The aim of this review is to provide an overview of the state of this field in plants. We review the history and uses of cytogenetic maps, and discuss future directions based on what we have learned.