Effect of muscle action and metabolic strain on oxidative metabolic responses in human skeletal muscle.
Effect of muscle action and metabolic strain on oxidative metabolic responses in human skeletal muscle.
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肌肉活动和代谢应变对人体骨骼肌氧化代谢反应的影响。
DOI：
10.1152/jappl.1999.87.5.1768
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Balaban,RS
中科院分区：
文献类型：
--
作者：
Combs,CA;Aletras,AH;Balaban,RS
最近的一份报告表明，人类肌肉的向心和偏心肌肉动作之间存在有氧能力差异（T. W. Ryschon、M. D. Fowler、R. E. Wysong、A. R. Anthony 和 R. S. Balaban.J. Appl. Physiol.83: 867–874, 1997）。本研究以磷酸肌酸 (PCr) 再合成率的形式比较了氧化反应，与体内任一肌肉动作锻炼的胫骨前肌中代谢应变的匹配水平（即 ADP 浓度或 ATP 水解自由能的变化）（n = 7 名受试者）。分别通过测力计和 31 P-磁共振波谱法控制运动并测量代谢应变。改变代谢菌株以使细胞质 ADP 浓度达到 55 μM 或将 ATP 水解自由能降低到 -55 kJ/mol，而细胞质 pH 值没有变化。运动后向心和离心动作的 PCr 再合成率分别为 31.9 至 462.5 和 21.4 至 405.4 μmol PCr/s。作为代谢应变函数的 PCr 再合成率在肌肉动作之间没有显着差异 (P> 0.40)，表明氧化能力取决于代谢应变，而不是肌肉动作。当将代谢应变增加氧化能力的术语添加到呼吸控制模型中时，发现汇总数据更符合之前的生化测量结果。
A recent report suggests that differences in aerobic capacity exist between concentric and eccentric muscle action in human muscle (T. W. Ryschon, M. D. Fowler, R. E. Wysong, A. R. Anthony, and R. S. Balaban.J. Appl. Physiol.83: 867–874, 1997). This study compared oxidative response, in the form of phosphocreatine (PCr) resynthesis rates, with matched levels of metabolic strain (i.e., changes in ADP concentration or the free energy of ATP hydrolysis) in tibialis anterior muscle exercised with either muscle action in vivo (n= 7 subjects). Exercise was controlled and metabolic strain measured by a dynamometer and31P-magnetic resonance spectroscopy, respectively. Metabolic strain was varied to bring cytosolic ADP concentration up to 55 μM or decrease the free energy of ATP hydrolysis to −55 kJ/mol with no change in cytoplasmic pH. PCr resynthesis rates after exercise ranged from 31.9 to 462.5 and from 21.4 to 405.4 μmol PCr/s for concentric and eccentric action, respectively. PCr resynthesis rates as a function of metabolic strain were not significantly different between muscle actions (P> 0.40), suggesting that oxidative capacity is dependent on metabolic strain, not muscle action. Pooled data were found to more closely conform to previous biochemical measurements when a term for increasing oxidative capacity with metabolic strain was added to models of respiratory control.