Inositol-requiring mutants of Saccharomyces cerevisiae.
Inositol-requiring mutants of Saccharomyces cerevisiae.
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酿酒酵母的肌醇需求突变体。
DOI：
10.1093/genetics/80.1.23
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发表时间：
1975
期刊：
影响因子：
3.3
通讯作者：
S. Henry
中科院分区：
文献类型：
--
作者：
M. Culbertson;S. Henry
用甲磺酸乙酯诱变后分离出52株需要肌醇的酿酒酵母突变体。互补和四分体分析揭示了10个主要的互补类，代表了10个独立分离的位点（命名为ino1至ino10），它们与各自的着丝粒自由重组。任何给定互补类的成员作为单个位点的等位基因分离。在36个突变体中鉴定出13个互补亚类，这些亚类表现为ino1位点的等位基因。这些突变体的互补图谱呈圆形。-在肌醇饥饿条件下，在具有代表性的突变体的液体培养中观察到细胞活力急剧下降，表明生长不平衡。对细胞死亡时间、动力学和程度的研究表明，在2-4 log数量级范围内的细胞活力损失可以通过向培养基中添加肌醇或用环己亚胺破坏蛋白质合成来预防。在本研究中发现的十个基因座中有九个有缺陷的突变体表现出这些不寻常的特征。结果表明，肌醇具有重要的生理作用，可能与其在细胞膜磷脂中的细胞定位和功能有关。本文讨论了肌醇缺乏通过丧失生物膜的正常组装、功能或完整性而引发不平衡生长过程导致细胞死亡的可能性。-这项工作的一部分已以初步形式报告（CULBERTSON和HENRY 1974）。
Fifty-two inositol-requiring mutants of Saccharomyces cerevisiae were isolated following mutagenesis with ethyl methanesulfonate. Complementation and tetrad analysis revealed ten major complementation classes, representing ten independently segregating loci (designated ino1 through ino10) which recombined freely with their respective centromeres. Members of any given complementation class segregated as alleles of a single locus. Thirteen complementation subclasses were identified among thirty-six mutants which behaved as alleles of the ino1 locus. The complementation map for these mutants was circular. - Dramatic cell viability losses indicative of unbalanced growth were observed in liquid cultures of representative mutants under conditions of inositol starvation. Investigation of the timing, kinetics, and extent of cell death revealed that losses in cell viability in the range of 2-4 log orders could be prevented by the addition of inositol to the medium or by disruption of protein synthesis with cycloheximide. Mutants defective in nine of the ten loci identified in this study displayed these unusual characteristics. The results suggest an important physiological role for inositol that may be related to its cellular localization and function in membrane phospholipids. The possibility is discussed that inositol deficiency initiates the process of unbalanced growth leading to cell death through the loss of normal assembly, function, or integrity of biomembranes. - Part of this work has been reported in preliminary form (CULBERTSON and HENRY 1974).
酿酒酵母温度敏感的葡萄糖胺营养缺陷型。
影响因子：
5.3
作者：
Ballou,L;Grove,JR;Roon,RJ;Wiggs,J;Ballou,CE
通讯作者：
Ballou,CE