Existence of GPR40 functioning in a human breast cancer cell line, MCF-7
Existence of GPR40 functioning in a human breast cancer cell line, MCF-7
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DOI：
10.1016/j.bbrc.2003.12.175
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发表时间：
2004-02-13
影响因子：
3.1
通讯作者：
Obara, Y
中科院分区：
文献类型：
--
作者：
Yonezawa, T;Katoh, K;Obara, Y
GPR40最近被确定为长链脂肪酸的g蛋白偶联细胞表面受体，在人乳腺癌细胞系（MCF-7）中进行了评估。我们通过RT-PCR检测GPR40 mRNA，发现油酸盐和亚油酸盐，而不是棕榈酸盐或硬脂酸盐，引起细胞Ca2+浓度的增加，这被百日咳毒素（PTX）治疗部分阻断。通过定量RT-PCR检测GPR40 mRNA的表达与细胞数量的关系。在细胞增殖初期和增殖末期均显著升高。这些结果提示长链脂肪酸的GPR40可能参与细胞增殖等细胞功能，为长链脂肪酸对乳腺上皮细胞的作用提供了新的视角。(C) 2003 Elsevier Inc.版权所有。
GPR40, which has recently been identified as a G-protein-coupled cell-surface receptor for long-chain fatty acids, was assessed in a human breast cancer cell line (MCF-7). We detected GPR40 mRNA by RT-PCR and found that oleate and linoleate, but not palmitate or stearate, caused an increase in cellular Ca2+ concentrations, which was partially blocked by the pertussis toxin (PTX) treatment. We examined the expression of GPR40 mRNA by quantitative RT-PCR in the relation to cell number. It was significantly increased at the beginning and at the end of cell proliferation. These results indicate the possibility that GPR40 for long-chain fatty acids may be involved in cellular function such as cell proliferation, providing a new perspective for the action of long-chain fatty acids on mammary epithelial cells. (C) 2003 Elsevier Inc. All rights reserved.