Sperm mitochondrial DNA depletion in men with asthenospermia
Sperm mitochondrial DNA depletion in men with asthenospermia
复制标题
DOI：
10.1016/j.fertnstert.2003.11.056
复制
发表时间：
2004-07-01
影响因子：
6.7
通讯作者：
Wei, YH
中科院分区：
文献类型：
--
作者：
Kao, SH;Chao, HT;Wei, YH
目的：测定弱精子症或精子活动力差患者精子线粒体DNA（mtDNA）含量。设计：采用一种新的同步聚合酶链反应分析mtDNA含量，即mtDNA和核DNA含量的比值。地点：大学医院不孕症中心。患者：86名寻求不孕症治疗的男性。干预措施：采用计算机辅助精液分析仪检测精子的运动特征。主要观察指标：精子样本分为弱精子症组和运动特征正常组。聚合酶链反应测定精子线粒体DNA含量。结果：弱精子症和精子活动力低下患者精子线粒体DNA含量明显降低，精子活力低下患者精子线粒体DNA含量明显降低，精子活力低下患者精子线粒体DNA含量明显降低，精子活力低下患者精子线粒体DNA含量明显降低。
Objective: To determine the content of sperm mitochondrial DNA (mtDNA) in patients with asthenospermia or with poor sperm motility.Design: Analysis of the content of mtDNA as the ratio between the amount of mtDNA and nuclear DNA by using a new concurrent polymerase chain reaction.Setting: University hospital infertility center.Patient(s): Eighty-six men who sought infertility therapy.Intervention(s): Moving characteristics of sperm were examined with a computer-assisted semen analyzer.Main Outcome Measure(s): Sperm samples were classified into two groups, one with asthenospermia and the other with normal moving characteristics. The content of mtDNA in sperm was determined by polymerase chain reaction. We analyzed the mitochondrial mass by MitoTracker Green staining and analyzed DNA content with propidium iodide staining by flow cytometry.Result(s): A decrease in sperm mtDNA content was detected in patients with asthenospermia or with poor sperm motility (