MIiSR: Molecular Interactions in Super-Resolution Imaging Enables the Analysis of Protein Interactions, Dynamics and Formation of Multi-protein Structures.
MIiSR: Molecular Interactions in Super-Resolution Imaging Enables the Analysis of Protein Interactions, Dynamics and Formation of Multi-protein Structures.
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DOI：
10.1371/journal.pcbi.1004634
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发表时间：
2015-12
影响因子：
4.3
通讯作者：
Heit B
中科院分区：
文献类型：
--
作者：
Caetano FA;Dirk BS;Tam JH;Cavanagh PC;Goiko M;Ferguson SS;Pasternak SH;Dikeakos JD;de Bruyn JR;Heit B
我们目前的理解，调节细胞过程，如囊泡运输的分子机制，已使常规的生化和显微镜技术。然而，这些方法往往掩盖了异质性的细胞环境，从而排除了定量评估的分子相互作用调节这些过程。在这里，我们提出了超分辨率（MIiSR）软件中的分子相互作用，该软件提供了用于超分辨率图像的定量分析工具。MIiSR结合了多种工具，用于分析分子间相互作用，分子聚类和图像分割。这些工具能够在细胞的天然环境中定量分子相互作用和高阶分子复合物的形成。这些分析工具的能力和局限性，证明使用模型化的数据和来自囊泡运输系统的例子，从而提供了一个建立和验证的实验工作流程，能够定量评估分子间的相互作用和分子复合物的形成内的异质环境的细胞。在本文中，我们提出了软件包分子相互作用在超分辨率（MIiSR），它提供了一系列的定量分析工具，用于测量分子间的相互作用和形成的高阶分子复合物在超分辨率显微镜图像。
Our current understanding of the molecular mechanisms which regulate cellular processes such as vesicular trafficking has been enabled by conventional biochemical and microscopy techniques. However, these methods often obscure the heterogeneity of the cellular environment, thus precluding a quantitative assessment of the molecular interactions regulating these processes. Herein, we present Molecular Interactions in Super Resolution (MIiSR) software which provides quantitative analysis tools for use with super-resolution images. MIiSR combines multiple tools for analyzing intermolecular interactions, molecular clustering and image segmentation. These tools enable quantification, in the native environment of the cell, of molecular interactions and the formation of higher-order molecular complexes. The capabilities and limitations of these analytical tools are demonstrated using both modeled data and examples derived from the vesicular trafficking system, thereby providing an established and validated experimental workflow capable of quantitatively assessing molecular interactions and molecular complex formation within the heterogeneous environment of the cell. In this paper we present the software package Molecular Interactions in Super Resolution (MIiSR), which provides a series of quantitative analytical tools for measuring molecular interactions and the formation of higher-order molecular complexes in super-resolution microscopy images.